Granulocyte macrophage colony-stimulating factor in adenomyosis and autologous endometrium.
Granulocyte macrophage colony-stimulating factor in adenomyosis and autologous endometrium.
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DOI：
10.1016/s1071-5576(01)00160-5
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发表时间：
2002-03
期刊：
影响因子：
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通讯作者：
A. Propst;B. Quade;R. Nowak;E. Stewart
中科院分区：
文献类型：
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作者：
A. Propst;B. Quade;R. Nowak;E. Stewart
目的：粒细胞巨噬细胞集落刺激因子（GM-CSF）与巨噬细胞的招募和激活有关，并已在人类子宫内膜中得到鉴定。我们确定子宫腺肌症是否表达 GM-CSF，如果存在，则比较子宫腺肌症与自体子宫内膜中 GM-CSF 蛋白的表达。方法：我们检查了 16 名因异常子宫出血、盆腔疼痛或子宫脱垂而接受子宫切除术的绝经前妇女的异位和在位子宫内膜。通过免疫组织化学分析含有子宫内膜和子宫腺肌症的绝经前子宫的连续切片中的 GM-CSF 配体和受体以及 CD68 巨噬细胞。我们分析了子宫腺肌病和自体子宫内膜的腺上皮和间质中GM-CSF配体和受体以及巨噬细胞的染色强度。结果：GM-CSF配体主要定位于腺上皮和子宫肌层，仅有浅间质染色。与自体子宫内膜腺体相比，子宫腺肌腺体中 GM-CSF 配体的染色明显更高 (P = .002)，特别是在月经周期的分泌期。子宫腺肌症和自体子宫内膜中GM-CSF受体的染色量和强度没有统计学差异。子宫腺肌组织比匹配的自体子宫内膜含有明显更多的巨噬细胞（P = .0004）。结论：与绝经前女性的自体子宫内膜相比，子宫腺肌腺上皮的 GM-CSF 配体表达量更高，这表明 GM-CSF 可能在增加子宫腺肌病女性的活化巨噬细胞水平方面发挥作用。
Objective:Granulocyte macrophage colony-stimulating factor (GM-CSF) has been related to macrophage recruitment and activation and has been identified in the human endometrium. We determined whether adenomyosis expresses GM-CSF, and if present, compared GM-CSF protein expression in adenomyosis with that in autologous endometrium.Methods:We examined ectopic and eutopic endometrium from 16 premenopausal women who had hysterectomies for abnormal uterine bleeding, pelvic pain, or uterine prolapse. Serial sections of premenopausal uteri containing endometrium and adenomyosis were analyzed by immunohistochemistry for GM-CSF ligand and receptor and CD68 macrophages. We analyzed the intensity of staining for GM-CSF ligand and receptor and macrophages in the glandular epithelium and stroma of adenomyosis and autologous endometrium.Results:The GM-CSF ligand localized primarily in the glandular epithelium and myometrium with only light stromal staining. Staining for GM-CSF ligand was significantly higher in adenomyotic glands compared with autologous endometrial glands (P = .002), especially during the secretory phase of the menstrual cycle. There were no statistical differences in the amount and intensity of staining of the GM-CSF receptor in adenomyosis and autologous endometrium. Adenomyotic tissue contained significantly more macrophages than matched autologous endometrium (P = .0004).Conclusions:Adenomyotic glandular epithelium had greater expression of the GM-CSF ligand compared with autologous endometrium from premenopausal women, which indicates that GM-CSF may play a role in increasing the levels of activated macrophages in women with adenomyosis.