PRENATAL DEVELOPMENT OF THE INTRINSIC NEURONS OF THE RAT NEOCORTEX - A COMPARATIVE-STUDY OF THE DISTRIBUTION OF GABA-IMMUNOREACTIVE CELLS AND THE GABA-A RECEPTOR
PRENATAL DEVELOPMENT OF THE INTRINSIC NEURONS OF THE RAT NEOCORTEX - A COMPARATIVE-STUDY OF THE DISTRIBUTION OF GABA-IMMUNOREACTIVE CELLS AND THE GABA-A RECEPTOR
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DOI：
10.1016/0306-4522(91)90127-a
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发表时间：
1991-01-01
期刊：
影响因子：
3.3
通讯作者：
SANCHEZ, MP
中科院分区：
文献类型：
--
作者：
COBAS, A;FAIREN, A;SANCHEZ, MP
用免疫细胞化学方法研究了出生前大鼠脑内GABA样免疫反应细胞的发育和GABA（A）受体免疫反应细胞的分布。 胚胎第14天，少数GABA样免疫反应（GABA阳性）细胞散在边缘区（原始丛状层）的软膜下界限在发育中的皮质的外侧部分。 GABA阳性细胞逐渐出现在原始丛状层的背侧和内侧部分，占据层内更深的位置。 GABA（A）受体的免疫反应性覆盖整个原始网状层。 到胚胎第16天，大多数GABA阳性细胞填充三个不同的层状隔室的发展中的皮质：预期的板I，基板，和中间区的下部。 下中间区的GABA阳性细胞似乎是典型的发育中的大鼠大脑皮层;其神经元的性质进行了免疫细胞化学评估，使用单克隆抗体对微管相关蛋白2，主要表达在神经元胞体和树突，对中间丝蛋白波形蛋白，表达在神经胶质细胞。 较低的中间区包含微管相关蛋白2的免疫反应性细胞，虽然免疫染色强度低于预期的板I和subplate。 初步结果显示，在下中间区没有波形蛋白阳性细胞。 在胚胎第16天，GABA（A）受体的免疫反应性存在于预期的板层I和亚板，但在下中间区没有。 从胚胎第18天开始，GABA（A）受体的免疫染色标记，明确，亚板作为一个薄层，清楚地从juvenile皮质板分离。 在胚胎第18天，GABA（A）受体开始在皮质板的下部分化部分表达。 在皮质板内，GABA在神经细胞胞体中的表达和GABA（A）受体的免疫染色在出生前和出生后早期具有相似的时空梯度（“由内向外”），大多数下中间带的GABA阳性细胞在胚胎20天开始消失（或停止表达GABA），但仍有一些细胞保留到成年。 一个类似的时间过程中观察到的微管相关蛋白2-免疫反应细胞群位于同一水平。假设在这里，在大鼠的大脑皮层，GABA样免疫反应细胞的下中间区可能有助于成人白色物质的间质细胞的人口，而基板仍然在成人的第六b层。
The ontogenesis of cells showing GABA-like immunoreactivity, and the distribution of the immunoreactivity for the GABA(A) receptor were studied immunocytochemically in the prenatal rat brain. By embryonic day 14, a few GABA-like immunoreactive (GABA-positive) cells scattered at the subpial limit of the marginal zone (primordial plexiform layer) in the lateral part of the developing cortex. GABA-positive cells appeared progressively within the dorsal and medial sectors of the primordial plexiform layer, occupying deeper positions within the layer. The immunoreactivity for the GABA(A) receptor covered the whole thickness of the primordial plexiform layer. By embryonic day 16, most GABA-positive cells populated three distinct laminar compartments of the developing cortex: the prospective lamina I, the subplate, and the lower part of the intermediate zone. The GABA-positive cells of the lower intermediate zone appeared to be typical of the developing cerebral cortex of the rat; their neuronal nature was assessed immunocytochemically, using monoclonal antibodies against microtubule-associated protein 2, mainly expressed in neuronal somata and dendrites, and against intermediate filament protein vimentin, expressed in glia. The lower intermediate zone contained cells immunoreactive for microtubule-associated protein 2, although the immunostaining was less intense than in the prospective lamina I and the subplate. Preliminary results showed no vimentin-positive cells in the lower intermediate zone. At embryonic day 16, immunoreactivity for the GABA(A) receptor was present within the prospective lamina I and the subplate, but not in the lower intermediate zone. From embryonic day 18 onwards, the immunostaining for the GABA(A) receptor labelled, unambiguously, the subplate as a lamina clearly separated from the suprajacent cortical plate. At embryonic day 18, the GABA(A) receptor started to be expressed within the lower, differentiating part of the cortical plate. Within the cortical plate, the expression of GABA in neural cell perikarya, and the immunostaining for the GABA(A) receptor, followed a similar spatio-temporal ("inside-out") gradient during pre- and early postnatal stages.Most GABA-positive cells of the lower intermediate zone started to disappear (or stopped the expression of GABA) by embryonic day 20, but some remained until adulthood. A similar time-course was observed for the microtubule-associated protein 2-immunoreactive cell population located at the same level.It is hypothesized here that, in the cerebral cortex of the rat, the GABA-like immunoreactive cells of the lower intermediate zone may contribute to the population of interstitial cells of the adult white matter, while the subplate remains in the adult as layer VIb.