Electrochemical detection of lipophilic antioxidants with high sensitivity at boron-doped diamond electrode
Electrochemical detection of lipophilic antioxidants with high sensitivity at boron-doped diamond electrode
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DOI：
10.1016/j.electacta.2013.02.052
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发表时间：
2013-04
影响因子：
6.6
通讯作者：
T. Kondo;K. Sakai;Takeshi Watanabe;Y. Einaga;M. Yuasa
中科院分区：
文献类型：
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作者：
T. Kondo;K. Sakai;Takeshi Watanabe;Y. Einaga;M. Yuasa
采用循环伏安法（CV）和流动注射电化学方法研究了亲脂性抗氧化剂α-生育酚（维生素E，VE）和泛醌（辅酶Q10，CoQ 10）在掺硼金刚石（BDD）电极上的电化学检测。循环伏安测试结果表明，VE和辅酶Q10在氧化BDD电极表面的电化学反应过电位大于在氢化BDD（H-BDD）电极表面的电化学反应过电位.用于测定VE和CoQ 10浓度的CV校准曲线的斜率在玻碳（GC）电极上比在H-BDD电极上大;然而，由于H-BDD电极的低背景特征，H-BDD电极上的信号背景比在GC电极上大。检测限（LOD），由流动注射电化学检测的信噪比为3定义，在H-BDD电极（VE：41 nM和辅酶Q10：17 nM）比在GC电极（VE：263 nM和辅酶Q10：71 nM）小得多。这些结果证实了H-BDD电极对于亲脂性抗氧化剂的电化学检测是可靠的，特别是在低浓度下。
The electrochemical detection of lipophilic antioxidants, α-tocopherol (vitamin E, VE) and ubiquinone (coenzyme Q10, CoQ10), in methanol and in methanol/hexane mixture solutions, respectively, at boron-doped diamond (BDD) electrodes was investigated by cyclic voltammetry (CV) and flow-injection electrochemical measurements. CV measurements revealed that the overpotential for electrochemical reaction of VE and CoQ10was larger at an oxidized BDD electrode surface than at that of a hydrogenated BDD (H-BDD) electrode. The slope of the CV calibration curve used to determine the VE and CoQ10concentrations was larger at a glassy carbon (GC) electrode than at the H-BDD electrode; however, the signal-to-background ratio was larger at the H-BDD electrode than at the GC electrode, due to the low background feature of the H-BDD electrode. The limit of detection (LOD), defined by a signal-to-noise ratio of three for flow-injection electrochemical detection, was much smaller at the H-BDD electrode (VE: 41nM and CoQ10: 17nM) than at the GC electrode (VE: 263nM and CoQ10: 71nM). These results confirm that the H-BDD electrode is reliable for the electrochemical detection of lipophilic antioxidants, especially at low concentrations.