Caspase-1-responsive fluorescence biosensors for monitoring endogenous inflammasome activation.
Caspase-1-responsive fluorescence biosensors for monitoring endogenous inflammasome activation.
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DOI：
10.1016/j.bios.2022.114812
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发表时间：
2022-10
影响因子：
12.6
通讯作者：
Xingxing Ren;Menglin Tao;Xiaoming Liu;Lele Zhang;Mingsong Li;Zijuan Hai
中科院分区：
文献类型：
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作者：
Xingxing Ren;Menglin Tao;Xiaoming Liu;Lele Zhang;Mingsong Li;Zijuan Hai
重点开发了两种特异性监测炎性小体激活的caspase-1响应荧光生物传感器。肽底物WEHD比YVAD更适合体外对caspase-1敏感和特异的反应。WEHD-HCy比YVAD-HCy更有利于体内监测常见炎症性疾病模型中的caspase-1活性。
HighlightsTwo caspase-1-responsive fluorescence biosensors to specifically monitor the activation of inflammasome were developed.The peptide substrate WEHD is more optimal than YVAD for sensitive and specifical response to caspase-1 in vitro.WEHD-HCy is more advantageous than YVAD-HCy to monitor caspase-1 activity in the common inflammatory disease model in vivo.