Fruit-localized phytochromes regulate lycopene accumulation independently of ethylene production in tomato
Fruit-localized phytochromes regulate lycopene accumulation independently of ethylene production in tomato
复制标题
DOI：
10.1104/pp.123.1.363
复制
发表时间：
2000-05-01
期刊：
影响因子：
7.4
通讯作者：
Pratt, LH
中科院分区：
文献类型：
--
作者：
Alba, R;Cordonnier-Pratt, MM;Pratt, LH
我们发现光敏色素对光诱导番茄果实（Solanum lycopersicum L.）成熟的各个方面进行差异调节。 Northern 分析表明，果实中的光敏色素 A mRNA 在成熟过程中积累了 11.4 倍。果皮组织的光谱辐射测量表明，从未成熟的绿色到红熟阶段的成熟过程中，果皮组织中的红色与远红色的比率增加了4倍。对收获的成熟绿色果实进行短暂红光处理可在果实发育过程中刺激番茄红素积累 2.3 倍。这种红光诱导的番茄红素积累通过随后的远红光处理被逆转，证实番茄中光诱导的番茄红素积累受到果实局部光敏色素的调节。成熟过程中的红光和红光/远红光处理不影响乙烯的产生，表明这些组织中这种成熟激素的生物合成不受果实局部光敏色素的调节。用红光或红/远红光处理的果实的压缩分析表明，光敏色素不调节成熟过程中果皮软化的速率或程度。此外，用红光或红光/远红光处理不会改变水果中柠檬酸盐、苹果酸盐、果糖、葡萄糖或蔗糖的浓度。这些结果与两个结论一致：（a）果实局部光敏色素独立于乙烯生物合成调节光诱导的番茄红素积累； (b) 水果局部光敏色素不是成熟的全局调节剂，而是调节这一发育过程的一个或多个特定组成部分。
We show that phytochromes modulate differentially various facets of light-induced ripening of tomato fruit (Solanum lycopersicum L.). Northern analysis demonstrated that phytochrome A mRNA in fruit accumulates 11.4-fold during ripening. Spectroradiometric measurement of pericarp tissues revealed that the red to far-red ratio increases 4-fold in pericarp tissues during ripening from the immature-green to the red-ripe stage. Brief red-light treatment of harvested mature-green fruit stimulated lycopene accumulation 2.3-fold during fruit development. This red-light-induced lycopene accumulation was reversed by subsequent treatment with far-red light, establishing that light-induced accumulation of lycopene in tomato is regulated by fruit-localized phytochromes. Red-light and red-light/far-red-light treatments during ripening did not influence ethylene production, indicating that the biosynthesis of this ripening hormone in these tissues is not regulated by fruit-localized phytochromes. Compression analysis of fruit treated with red light or red/far-red light indicated that phytochromes do not regulate the rate or extent of pericarp softening during ripening. Moreover, treatments with red or red/far-red light did not alter the concentrations of citrate, malate, fructose, glucose, or sucrose in fruit. These results are consistent with two conclusions: (a) fruit-localized phytochromes regulate light-induced lycopene accumulation independently of ethylene biosynthesis; and (b) fruit-localized phytochromes are not global regulators of ripening, but instead regulate one or more specific components of this developmental process.