First report of Puccinia crepidis-japonicae in Puerto Rico, causing rust disease in oriental false hawksbeard
First report of Puccinia crepidis-japonicae in Puerto Rico, causing rust disease in oriental false hawksbeard
复制标题
波多黎各首次报道引起东方假鹰须锈病的锈病
DOI：
10.1007/s42161-022-01300-1
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发表时间：
2023
影响因子：
2.2
通讯作者：
Poindexter, Tara
中科院分区：
文献类型：
--
作者：
Savchenko, Kyryll;Poindexter, Tara
杨藤（菊科）是一种一年生的世界性草本植物，通常被称为东方假鹰须，生长在受干扰的地区。它原产于东亚（中国和日本），被认为是非洲、美洲和澳大利亚-大洋洲的入侵物种。在中国，叶子被用来治疗感冒、喉咙痛和腹泻。亚洲、美洲和大洋洲均报道过该宿主的日本信用锈菌（Farr and Rossman 2021）。2022年5月，在波多黎各Cerro de Punta山顶附近的日本稻（Y. japonica）叶片上观察到由一种锈病引起的锈病。大约60%的观察到的植物被感染。对标本进行了显微镜检查，并与已发表文献中的模式标本描述进行了比较。尿膜雌雄同体，圆形，裸露，散在，直径小于0.5 mm，呈褐色。孢子球形至椭球状，黄棕色，直径19.9 ~ 21.9× 22-25 μm (n= 20)，棘刺状，细胞壁约2 μm，深黄褐色，有2 ~ 3个胚芽孔。没有看到telia。采用Omega公司的EZNA Plant DNA试剂盒从单个锈菌中提取DNA。内部转录间隔区（ITS）和大亚基区（LSU）分别按照Demers等人（2017）和Aime等人（2018）的方案进行扩增。序列保存在GenBank中（accession no . ON938164, ON921007）。BLASTn检索结果显示，该序列与日本血吸虫上P. crepidis-japonicae的ITS （KY798395）和LSU （KY798360）序列具有100%的核苷酸相似性。在genous Prime平台上利用贝叶斯方法对连接的数据进行系统发育分析，进一步确认该物种为p.c repidis-japonicae。据我们所知，这是波多黎各首次报道的日本信贷假单胞虫。作为杂草寄主的病原菌，crepidis-japonicae可能在对日本青花的生物防治中发挥重要作用。
Youngia japonica (Asteraceae) is an annual, cosmopolitan herb, commonly known as oriental false hawksbeard, that inhabits disturbed areas. It is native to eastern Asia (China and Japan), and is considered invasive in Africa, the Americas, and Australia-Oceania. Leaves are used as a remedy of cold, sore throat, and diarrhea in China. Puccinia crepidis-japonicae was reported on this host from Asia, the Americas, and Oceania (Farr and Rossman 2021). In May 2022, rust disease caused by a Puccinia species was observed on leaves of Y. japonica near the summit of Cerro de Punta, Puerto Rico. Roughly 60% of observed plants were infected. The specimens were examined microscopically and compared with type specimen descriptions in published literature. The uredinia were amphigenous, rounded, naked, scattered, less than 0.5 mm in diameter, brownish. Urediniospores were globose to ellipsoid, yellow-brown, 19.9–21.9× 22–25 μm (n= 20), echinulate, cell walls around 2 μm, dark yellow brown, with two to three germ pores. No telia were seen. DNA was extracted from a single rust sorus using EZNA Plant DNA kit by Omega. The internal transcribed spacer (ITS), and large subunit (LSU) regions were amplified following the protocols of Demers et al.(2017) and Aime et al.(2018), respectively. Sequences were deposited in GenBank (accession Nos. ON938164, ON921007). A BLASTn search revealed that the sequences share 100% nucleotide similarities with the ITS (KY798395) and LSU (KY798360) sequences of P. crepidis-japonicae on Y. japonica. Phylogenetic analysis of the concatenated dataset using the Bayesian method performed on the Geneious Prime platform, further confirmed the species as P. crepidis-japonicae. To our knowledge, this is the first report of P. crepidis-japonicae in Puerto Rico. Being a pathogen of weedy hosts, P. crepidis-japonicae may play a significant role as a biocontrol agent against Youngia japonica.