MOLECULAR-CLONING, CHARACTERIZATION, AND EXPRESSION OF HUMAN ADP-RIBOSYLATION FACTORS - 2 GUANINE NUCLEOTIDE-DEPENDENT ACTIVATORS OF CHOLERA-TOXIN
MOLECULAR-CLONING, CHARACTERIZATION, AND EXPRESSION OF HUMAN ADP-RIBOSYLATION FACTORS - 2 GUANINE NUCLEOTIDE-DEPENDENT ACTIVATORS OF CHOLERA-TOXIN
复制标题
DOI：
10.1073/pnas.86.16.6101
复制
发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
VAUGHAN, M
中科院分区：
文献类型：
--
作者：
BOBAK, DA;NIGHTINGALE, MS;VAUGHAN, M
ADP核糖基化因子（ARF）是一种能够增强霍乱毒素酶活性的小分子鸟嘌呤核苷酸结合蛋白。从人小脑文库中克隆了两个ARF cDNA，即ARF 1和ARF 3。基于推导的氨基酸序列和cDNA和寡核苷酸探针与哺乳动物脑poly（A）+ RNA的杂交模式，人ARF 1是牛ARF 1的同源物。人ARF 3不同于牛ARF 1和牛ARF 2，似乎代表了新发现的第三种ARF。人ARF cDNA和克隆特异性寡核苷酸与poly（A）+ RNA的杂交模式与人脑中至少存在两种，也许是四种单独的ARF信息一致。通过SDS/PAGE，ARF 1、ARF 2和ARF 3的体外翻译产生的蛋白质的行为与纯化的可溶性脑ARF相似。推导出的人ARF 1和ARF 3的氨基酸序列含有与其他G蛋白相似的区域，这些区域被认为参与GTP结合和水解。ARF还表现出与牛磷脂酶C的适度同源性。这里报告的意见支持的结论，ARF是一个多基因家族的小鸟嘌呤核苷酸结合蛋白的成员。对ARF mRNA的调节和重组ARF蛋白的功能的定义将有助于阐明ARF的生理作用。
ADP-ribosylation factors (ARFs) are small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin. Two ARF cDNAs, ARF1 and ARF3, were cloned from a human cerebellum library. Based on deduced amino acid sequences and patterns of hybridization of cDNA and oligonucleotide probes with mammalian brain poly(A)+ RNA, human ARF1 is the homologue of bovine ARF1. Human ARF3, which differs from bovine ARF1 and bovine ARF2, appears to represent a newly identified third type of ARF. Hybridization patterns of human ARF cDNA and clone-specific oligonucleotides with poly(A)+ RNA are consistent with the presence of at least two, and perhaps four, separate ARF messages in human brain. In vitro translation of ARF1, ARF2, and ARF3 produced proteins that behaved, by SDS/PAGE, similar to a purified soluble brain ARF. Deduced amino acid sequences of human ARF1 and ARF3 contain regions, similar to those in other G proteins, that are believed to be involved in GTP binding and hydrolysis. ARFs also exhibit a modest degree of homology with a bovine phospholipase C. The observations reported here support the conclusion that the ARFs are members of a multigene family of small guanine nucleotide-binding proteins. Definition of the regulation of ARF mRNAs and of function(s) of recombinant ARF proteins will aid in the elucidation of the physiologic role(s) of ARFs.