Cryopreservation and Transplantation of Spermatogonial Stem Cells
Cryopreservation and Transplantation of Spermatogonial Stem Cells
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精原干细胞的冷冻保存和移植
DOI：
10.1007/978-1-0716-0970-5_4
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发表时间：
2021
影响因子：
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通讯作者：
Akos Horvath
中科院分区：
文献类型：
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作者：
Zoran Marinovic;Jelena Lujic;;Qian Li;Yoshiko Iwasaki;Bela Urbanyi;Goro Yoshizaki;Akos Horvath
低温保存作为一种能够长期保存生物材料的方法，长期以来一直被用于保存宝贵的斑马鱼遗传资源。然而，目前，只有斑马鱼的精子可以成功地冷冻保存，而卵子和胚胎的冷冻保存方案尚未完全开发。生殖系干细胞（GSCs）移植已成为一种有利的方法，它可以绕过当前雌性遗传资源冷冻保存的问题，并可以通过替代生产来重建鱼类物种和品系。在这里，我们描述了精原干细胞（SSC）的冷冻保存所需的基本步骤，并通过SSC移植和替代生产在斑马鱼遗传资源的保护中利用。
Cryopreservation as a method that enables long-term storage of biological material has long been used for the conservation of valuable zebrafish genetic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current problem in cryopreservation of female genetic resources and can lead to reconstitution of fish species and lines through surrogate production. Here, we describe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.