Pluronic F127 as a cell encapsulation material: Utilization of membrane-stabilizing agents
Pluronic F127 as a cell encapsulation material: Utilization of membrane-stabilizing agents
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DOI：
10.1089/ten.2005.11.974
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发表时间：
2005-05-01
影响因子：
--
通讯作者：
Roberts, SC
中科院分区：
文献类型：
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作者：
Khattak, SF;Bhatia, SR;Roberts, SC
共聚物Pluronic F127（通用名，泊洛沙姆407）在水中的热可逆凝胶化使其成为细胞包封应用的独特候选物，无论是单独的还是促进细胞接种和附着在组织支架中。在15 - 20%（w/w）的浓度下，Pluronic F127（F127）水溶液在生理温度下胶凝。测定了F127对液体和凝胶制剂的人肝癌细胞（HepG 2）的活力和增殖的影响。通过台盼蓝测定经由血细胞计数法测量5天期间的细胞浓度和活力，并且在MTT和LDH测定中证实结果。使用0.1 - 5%（w/w）F127（液体），细胞增殖并在5天内保持高活力。然而，在10%（w/w）F127（液体）下，细胞活力显著降低，并且没有明显的细胞增殖。HepG 2细胞包封在浓度范围为15至20%（w/w）（凝胶）的F127中导致5天的完全细胞死亡。对于评价的HMEC-1（内皮）和L 6（肌肉）细胞系也是如此。细胞接种密度不影响细胞存活或增殖。向F127凝胶制剂中加入膜稳定剂（氢化可的松、葡萄糖和甘油）以提高细胞活力。类固醇氢化可的松表现出最显著的活力改善，从< 2%（单独F127）到> 70%（加入60 nM氢化可的松）。这些结果表明，补充有膜稳定剂的F127制剂可用作活细胞包封材料。此外，氢化可的松通常可用于促进多种包封材料的细胞活力。
Thermoreversible gelation of the copolymer Pluronic F127 (generic name, poloxamer 407) in water makes it a unique candidate for cell encapsulation applications, either alone or to promote cell seeding and attachment in tissue scaffolds. At concentrations of 15 - 20% (w/w), aqueous Pluronic F127 (F127) solutions gel at physiological temperatures. The effect of F127 on viability and proliferation of human liver carcinoma cells (HepG2) was determined for both liquid and gel formulations. Cell concentration and viability over a 5- day period were measured by the trypan blue assay via hemocytometry and results were confirmed in both the MTT and LDH assays. With 0.1 - 5% (w/w) F127 (liquid), cells proliferated and maintained high viability over 5 days. However, at 10% (w/w) F127 (liquid), there was a significant decrease in cell viability and no cell proliferation was evident. HepG2 cell encapsulation in F127 concentrations ranging from 15 to 20% (w/w) (gel) resulted in complete cell death by 5 days. This was also true for the HMEC-1 (endothelial) and L6 (muscle) cell lines evaluated. Cell- seeding density did not affect cell survival or proliferation. Membrane-stabilizing agents (hydrocortisone, glucose, and glycerol) were added to the F127 gel formulations to improve cell viability. The steroid hydrocortisone demonstrated the most significant improvement in viability, from < 2% (in F127 alone) to > 70% (with 60 nM hydrocortisone added). These results suggest that F127 formulations supplemented with membrane-stabilizing agents can serve as viable cell encapsulation materials. In addition, hydrocortisone may be generally useful in the promotion of cell viability for a wide range of encapsulation materials.