Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry: a technique to map plant metabolites within tissues at high spatial resolution
Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry: a technique to map plant metabolites within tissues at high spatial resolution
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DOI：
10.1093/jxb/erl139
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发表时间：
2007-03-01
影响因子：
6.9
通讯作者：
Clench, M. R.
中科院分区：
文献类型：
--
作者：
Burrell, M. M.;Earnshaw, C. J.;Clench, M. R.
基质辅助激光解吸电离质谱技术为植物组织中代谢物的分布分析提供了一种新的、强有力的工具。两种基质α-氰基-4-羟基肉桂酸（α-CHCA）和9-氨基吖啶提供了一种有用的组合，可以测量氨基酸、糖和磷酸化代谢物。结果表明，这些代谢产物的代表是不均匀分布在小麦种子在不同阶段的发展和温度胁迫下。
Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry provides a new and powerful tool to analyse the distribution of metabolites within plant tissues. The two matrices alpha-cyano-4-hydroxycinnamic acid (alpha-CHCA) and 9-aminoacridine provide a useful combination that allows the measurement of amino acids, sugars, and phosphorylated metabolites. Results are presented showing that representatives of these metabolites are unevenly distributed in wheat seeds at different stages of development and under temperature stress.