Comparison of turbidimetry with particle counting for the determination of human beta 2-microglobulin by latex immunoassay (LIA).
Comparison of turbidimetry with particle counting for the determination of human beta 2-microglobulin by latex immunoassay (LIA).
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通过乳胶免疫分析 (LIA) 测定人 β2-微球蛋白时比浊法与粒子计数法的比较。
DOI：
10.1016/0009-8981(82)90347-3
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
R. Lauwerys
中科院分区：
文献类型：
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作者：
A. Bernard;R. Lauwerys
尿液中&微球蛋白(&-m)的测定目前被认为是早期发现肾小管间质性肾炎最敏感的检测方法。血清中 &-m 的测定已被提议用于监测各种肿瘤、炎症和免疫性疾病的演变[11。我们最近描述了一种新的非同位素方法，用于测定生物液体中的 &-m [2]。这种方法称为乳胶免疫测定 (LIA)，基于 &-m 吸附有抗 &-m 免疫球蛋白的校准乳胶颗粒的凝集作用。如前所述[2]，LIA在灵敏度、精密度和准确度方面与放射免疫分析（RIA）相当，并且两种方法在测定尿液或血清中的&-m时获得了良好的相关性。与放射或酶免疫分析相比，LIA 的主要优点是其极其简单、快速和低成本。 Initially, the agglutination of latex particles was quantified by particle counting (PACIA) as proposed by Cambiaso et al [3], but we have found that the agglutination could also be evaluated by turbidimetry.颗粒计数读数的灵敏度约为比浊法的两倍。然而，后者可以精确测量低至 1 pg/l 的 a &-m 浓度，因此对于尿液或血清中的 &-m 测定具有足够的灵敏度。人们对比浊读数的兴趣是显而易见的，因为它需要临床化学实验室常用的仪器。本文的目的是比较颗粒计数和比浊法的性能，以评估 &-m LIA 中乳胶颗粒的凝集情况。比较了两种读数方法的精度和准确度。
The determination of & microglobulin(&-m) in urine is currently considered as the most sensitive test for the early detection of tubulo-interstitial nephritis. The determination of &-m in serum has been proposed for monitoring the evolution of various neoplastic, inflammatory and immunological diseases [11. We have recently described a new non-isotopic method for the determination of &-m in biological fluids [2]. This method, called latex immunoassay(LIA), is based on the agglutination by &-m of calibrated latex particles on which anti-&-m immunoglobulins have been adsorbed. As shown previously [2], LIA compares well with radioimmunoassay(RIA) in terms of sensitivity, precision and accuracy, and an excellent correlation was obtained between both methods for the determination of &-m in urine or serum. The main advantages of LIA over radio-or enzymeimmunoassay are its extreme simplicity, rapidity and low cost. Initially, the agglutination of latex particles was quantified by particle counting (PACIA) as proposed by Cambiaso et al [3], but we have found that the agglutination could also be evaluated by turbidimetry. The particle counting reading is about twice as sensitive as the turbidimetric method. However, the latter can measure with precision a &-m concentration as low as 1 pg/l and is therefore sufficiently sensitive for &-m determination in urine or serum. The interest in turbidimetric reading is obvious, since it requires an instrument commonly used in the clinical chemistry laboratory. The aim of this paper is to compare the performances of particle counting and turbidimetry to evaluate the agglutination of latex particles in the LIA of &-m. Both reading methods were compared with respect to their precision and accuracy.