Differences in the microstructure and biomechanical properties of the recurrent laryngeal nerve as a function of age and location.
Differences in the microstructure and biomechanical properties of the recurrent laryngeal nerve as a function of age and location.
复制标题
喉返神经的微观结构和生物力学特性随年龄和位置的变化而变化。
DOI：
10.1115/1.4027682
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
VandeGeest,JonathanP
中科院分区：
文献类型：
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作者：
Williams,MeganJ;Utzinger,Urs;Barkmeier-Kraemer,JulieM;VandeGeest,JonathanP
特发性单侧声带麻痹（UVP）是由喉返神经（RLN）损伤引起的，并导致说话，呼吸和吞咽困难。这种损伤可能发生在该神经中，因为它环绕主动脉弓，这是在一个动态的生物力学环境。本研究的目的是确定是否位置依赖的生物力学和微观结构特性的RLN是不同的仔猪与青春期猪。对来自8只青少年猪和6只小猪的喉返神经的颈部/远端和胸部/近端（靠近主动脉弓）区域进行分离，并在单轴张力下进行机械评估。在机械测试期间，在5%、10%和15%应变下收集双光子成像（二次谐波）数据。在每个应变水平下测定切向模量（TM）和应变能密度（W）。根据成像数据计算优选纤维角的平均模式和半高宽（FWHM，纤维张开的量度）。我们发现左喉返神经近段的TM、W和FWHM值明显大于远段（TM为18.51 MPa ± 1.22 vs 10.78 MPa ± 1.22，p < 0.001，W为0.046 MPa ± 0.01 vs 0.026 MPa ± 0.01，p < 0.003，15.52度± 1.00对比12.98度± 1.00，对于FWHM，p < 0.001）。左侧节段的TM和W大于右侧节段（TM为15.32 MPa ± 1.20 vs 11.80 MPa ± 1.20，p < 0.002; W为0.038 MPa ± 0.01 vs 0.028 MPa ± 0.01，p < 0.0001）。与青春期猪相比，仔猪的W更大（0.042 MPa ± 0.01 vs 0.025 MPa ± 0.01，p < 0.04）。左侧猪喉返神经的近端区域比远端区域更硬，并且具有更高程度的纤维张开。青少年猪的左侧RLN也显示出比右侧更高程度的应变硬化。这些差异可能是由于左喉返神经在主动脉弓周围形成环时所处的动态环境更大。
Idiopathic onset of unilateral vocal fold paralysis (UVP) is caused by damage to the recurrent laryngeal nerve (RLN) and results in difficulty speaking, breathing, and swallowing. This damage may occur in this nerve as it loops around the aortic arch, which is in a dynamic biomechanical environment. The goal of this study is to determine if the location-dependent biomechanical and microstructural properties of the RLN are different in piglets versus adolescent pigs. The neck/distal and thoracic/proximal (near the aortic arch) regions of the RLN from eight adolescent pigs and six piglets were isolated and mechanically assessed in uni-axial tension. Two-photon imaging (second harmonic) data were collected at 5%, 10%, and 15% strain during the mechanical test. The tangential modulus (TM) and the strain energy density (W) were determined at each level of strain. The mean mode of the preferred fiber angle and the full width at half maximum (FWHM, a measure of fiber splay) were calculated from the imaging data. We found significantly larger values of TM, W, and FWHM in the proximal segments of the left RLN when compared to the distal segments (18.51 MPa ± 1.22 versus 10.78 MPa ± 1.22, p < 0.001 for TM, 0.046 MPa ± 0.01 versus 0.026 MPa ± 0.01, p < 0.003 for W, 15.52 deg ± 1.00 versus 12.98 deg ± 1.00, p < 0.001 for FWHM). TM and W were larger in the left segments than the right (15.32 MPa ± 1.20 versus 11.80 MPa ± 1.20, p < 0.002 for TM, 0.038 MPa ± 0.01 versus 0.028 MPa ± 0.01, p < 0.0001 for W). W was larger in piglets when compared to adolescent pigs (0.042 MPa ± 0.01 versus 0.025 MPa ± 0.01, p < 0.04). The proximal region of the left porcine RLN is more stiff than the distal region and has a higher degree of fiber splay. The left RLN of the adolescent pigs also displayed a higher degree of strain stiffening than the right. These differences may develop as a result of the more dynamic environment the left RLN is in as it loops around the aortic arch.