Mouse chromosome 5.
Mouse chromosome 5.
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小鼠5号染色体。
DOI：
10.1007/bf00656487
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Stephenson,DA
中科院分区：
文献类型：
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作者：
Kozak,CA;Stephenson,DA
对哺乳动物基因组图谱和测序的广泛兴趣鼓励了高分辨率小鼠分子遗传连锁图谱的发展。对于这个模式系统，早期对遗传连锁图谱生成的兴趣是成功的育种计划的自然结果，这些育种计划产生了各种近交系和突变株系。由于这些繁育群体还保存了数百个形态发生和行为突变，第一个连锁图谱包含了形态和行为表型的基因。后来的版本增加了免疫标记和蛋白质多态，现在正在使用多位点物种间杂交快速定位分子标记。我们现在的目标是将来自这些不同的遗传杂交以及重组自交系、体细胞杂交和原位杂交的数据协调到每条染色体的多位点连锁图谱中，将这些图谱与细胞遗传学标记相关联，并为物理图谱的开发奠定基础。这份报告是这项工作的组成部分，也是小鼠染色体委员会(Chr)5的第一份报告。对于Chr 5，这项任务得到了一些形态发生标记上的序列的分子克隆的帮助，这些标记被用作锚点，以开发该染色体的最早图谱，特别是RD、GUS、W、Alb-1和ALP。这简化了纯分子标记构建的图谱与早期研究积累的数据的整合。在准备这个图谱的过程中，我们整理和评估了所有已发表的关于Chr 5遗传标记的数据，并将这些数据合并到一个修订的连锁图谱中，我们希望它将作为进一步开发这段小鼠基因组的骨干。
The expanded interest in mammalian genome mapping and sequencing has encouraged the development of a high-resolution molecular genetic linkage map of the mouse. For this model system, early interest in the generation of a genetic linkage map was a natural outgrowth of successful breeding programs which had produced various inbred strains and mutant stocks. Since these breeding stocks also preserved hundreds of morphogenetic and behavioral mutations, the first linkage maps comprised genes for morphological and behavioral phenotypes. Later versions added immunological markers and protein polymorphisms, and now molecular markers are being rapidly mapped using multilocus interspecies crosses. Our goal is now to reconcile data from these various genetic crosses as well as from analysis of recombinant inbred (RI) strains, somatic cell hybrids, and in situ hybridization into multilocus linkage maps for each chromosome, to correlate these maps with cytogenetic markers, and to lay the groundwork for the development of a physical map. This report is an integral part of this effort and represents the first report of the Committee for Mouse Chromosome (Chr) 5. For Chr 5, this task has been aided by the molecular cloning of sequences at a number of the morphogenetic markers which were used as anchor loci to develop the earliest maps of this chromosome, specifically rd, Gus, W, Alb-1, and Alp. This has simplified the integration of maps constructed of purely molecular markers with the data accumulated from earlier studies. In the process of preparing this map, we have collated and evaluated all published data dealing with genetic markers on Chr 5 and incorporated these data into a revised linkage map which, we hope, will serve as the backbone for further development of this segment of the mouse genome.