Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster.
Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster.
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DOI：
10.1534/g3.113.007591
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发表时间：
2013-09-04
期刊：
影响因子：
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通讯作者：
Cohen SM
中科院分区：
文献类型：
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作者：
Kugler JM;Chen YW;Weng R;Cohen SM
microRNA（miRNAs）是基因表达的转录后调节因子，可以作为缓冲剂来稳定基因调控网络。在这里，我们确定了两个miRNAs，是母方所需的正常胚胎原始生殖细胞发育的果蝇。来自miR-969和miR-9c突变母体的胚胎平均生殖细胞数量减少。有趣的是，这种减少与这种数量表型性状的方差增加相关。对一组独立的母体突变基因型的分析表明，生殖细胞数量的减少不一定会导致方差增加。我们的观察结果与miR-969和miR-9c有助于稳定控制细菌数量的过程的假设一致，支持表型稳健性。
MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression that may act as buffering agents to stabilize gene-regulatory networks. Here, we identify two miRNAs that are maternally required for normal embryonic primordial germ cell development in Drosophila melanogaster. Embryos derived from miR-969 and miR-9c mutant mothers had, on average, reduced germ cell numbers. Intriguingly, this reduction correlated with an increase in the variance of this quantitative phenotypic trait. Analysis of an independent set of maternal mutant genotypes suggests that reduction of germ cell number need not lead to increased variance. Our observations are consistent with the hypothesis that miR-969 and miR-9c contribute to stabilizing the processes that control germ number, supporting phenotypic robustness.