Role of hypoxanthine-guanine phosphoribosyltransferase in the salvage pathway of fairy chemicals in rice
Role of hypoxanthine-guanine phosphoribosyltransferase in the salvage pathway of fairy chemicals in rice
复制标题
次黄嘌呤鸟嘌呤磷酸核糖基转移酶在水稻神奇化学物质拯救途径中的作用
DOI：
10.1039/d3ob00026e
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
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作者：
38)Takemura;H.;Choi;J-H.;Fushimi;K.;Narikawa;R.;Kondo;M.;Wu;J.;Nelson;D. C.;Suzuki;T.;Ouchi;H.;Inai;M.;Hirai;H.;and Kawagishi;H.
2-氮杂次黄嘌呤(AHX)、咪唑-4-甲酰胺(ICA)和2-氮杂-8-氧基次黄嘌呤(AOH)是植物体内具有多种功能的分子。FCS的生物合成途径是由5-氨基咪唑-4-甲酰胺生物合成的一种新的嘌呤代谢途径。在这里，我们展示了嘌呤回收酶之一，次黄嘌呤-鸟嘌呤磷酸核糖转移酶(HGPRT)，识别AHX和AOH为底物。用酶法合成了两个新化合物AOH核糖核苷酸及其核糖核苷。通过质谱学、一维和二维核磁共振波谱和X-射线单晶衍射法对其结构进行了表征。这份报告证明了HGPRT的功能以及与水稻FCS生物合成相关的新的嘌呤代谢的存在。
Fairy chemicals (FCs), 2-azahypoxanthine (AHX), imidazole-4-carboxamide (ICA), and 2-aza-8-oxohypoxanthine (AOH), are molecules with many diverse functions in plants. The defined biosynthetic pathway for FCs is a novel purine metabolism in which they are biosynthesized from 5-aminoimidazole-4-carboxamide. Here, we show that one of the purine salvage enzymes, hypoxanthine-guanine phosphoribosyltransferase (HGPRT), recognizes AHX and AOH as substrates. Two novel compounds, AOH ribonucleotide and its ribonucleoside which are the derivatives of AOH, were enzymatically synthesized. The structures were determined by mass spectrometry, 1D and 2D NMR spectroscopy, and X-ray single-crystal diffraction analysis. This report demonstrates the function of HGPRT and the existence of novel purine metabolism associated with the biosynthesis of FCs in rice.