The NB protein of influenza B virus is not necessary for virus replication in vitro
The NB protein of influenza B virus is not necessary for virus replication in vitro
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DOI：
10.1128/jvi.77.10.6050-6054.2003
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发表时间：
2003-05-01
影响因子：
5.4
通讯作者：
Kawaoka, Y
中科院分区：
文献类型：
--
作者：
Hatta, M;Kawaoka, Y
B型流感病毒的Nb蛋白被认为具有离子通道的功能，因此有望在病毒复制中发挥重要作用。由于缺乏直接证据证明其在病毒生命周期中的绝对需求，我们通过反向遗传学产生了NB基因敲除病毒，并测试了它们在体外和体内的生长特性。不表达NB的突变体在细胞培养中的复制效率与野生型病毒一样高，而在小鼠中，与野生型病毒的发现相比，它们的生长受到限制。因此，Nb蛋白不是B型流感病毒在细胞培养中复制所必需的，但可以促进小鼠的有效生长。
The NB protein of influenza B virus is thought to function as an ion channel and therefore would be expected to have an essential function in viral replication. Because direct evidence for its absolute requirement in the viral life cycle is lacking, we generated NB knockout viruses by reverse genetics and tested their growth properties both in vitro and in vivo. Mutants not expressing NB replicated as efficiently as the wild-type virus in cell culture, whereas in mice they showed restricted growth compared with findings for the wild-type virus. Thus, the NB protein is not essential for influenza B virus replication in cell culture but promotes efficient growth in mice.