Th1/Th2 imbalance, measured by circulating and intracytoplasmic inflammatory cytokines - Immunological alterations in acute coronary syndrome and stable coronary artery disease
Th1/Th2 imbalance, measured by circulating and intracytoplasmic inflammatory cytokines - Immunological alterations in acute coronary syndrome and stable coronary artery disease
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DOI：
10.1111/j.1365-3083.2006.01816.x
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发表时间：
2006-09-01
影响因子：
3.7
通讯作者：
Soltesz, P.
中科院分区：
文献类型：
--
作者：
Szodoray, P.;Timar, O.;Soltesz, P.
描述外周免疫参数如何反映冠状动脉粥样硬化中动脉粥样硬化斑块的炎症改变。我们测量了一般炎症标志物 C 反应蛋白 (CRP) 和粒细胞活性、淋巴细胞亚群及其激活状态，评估了循环 Th1/Th2 型细胞因子和特定胞浆内细胞因子。我们研究了免疫参数与疾病结果和死亡率的关联。研究对象包括 33 名急性冠状动脉综合征 (ACS) 患者、62 名稳定型冠状动脉疾病 (CAD) 患者和 58 名健康对照者。通过流式细胞术定量外周血淋巴细胞亚群，使用酶联免疫吸附测定（ELISA）评估可溶性细胞因子和自身抗体，而细胞内染色后通过流式细胞术测量细胞内细胞因子水平。我们发现 ACS 与 CAD 中的 CRP 和粒细胞活性水平升高（分别为 P < 0.001，P = 0.017）。与对照组相比，ACS 中自然杀伤 (NK) 细胞百分比升高，而 T 细胞占淋巴细胞总数的百分比略有下降（分别为 P < 0.0001，P = 0.012）。当对激活标记 HLA-DR3 和 CD69(+) 进行染色时，两种形式的冠状动脉粥样硬化均显示出比对照显着更高的激活 T 细胞百分比（分别为 ACS：P < 0.0001，P = 0.002，CAD：P < 0.0001，P = 0.018）。事实证明，ACS 中的 IL-1、IL-4 和 IL-10 显着高于对照组（分别为 P = 0.036、P = 0.01、P < 0.0001）。在这两种疾病中，Th1 与 Th2 的比例都转向 Th1 占主导地位。一般促炎标记物和活化的 T 细胞都表示 CAD。精心策划的促炎症级联反应最终导致疾病的发展。
To describe how peripheral immune-parameters reflect the inflammatory alterations of the atherosclerotic plaques in coronary atherosclerosis. We measured general inflammatory markers C-reactive protein (CRP) and granulocyte activity, lymphocyte subpopulations and their state of activation, evaluated circulating Th1/Th2-type cytokines, and specific intracytoplasmic cytokines. We investigated the association of immune-parameters with disease outcome and mortality. Thirty-three patients with acute coronary syndrome (ACS), 62 with stable coronary artery disease (CAD) and 58 healthy controls were studied. Peripheral blood lymphocyte subgroups were quantified by flow cytometry, soluble cytokines and autoantibodies were assessed using enzyme-linked immunosorbent assay (ELISA), while intracellular cytokine levels were measured by flow cytometry after intracellular staining. We found elevated levels of CRP and granulocyte activity in ACS versus CAD (P < 0.001, P = 0.017, respectively). Natural killer (NK) cell percentages were elevated, while percentage of T cells to the total lymphocyte count was slightly decreased in ACS compared to controls (P < 0.0001, P = 0.012, respectively). Both forms of coronary atherosclerosis showed significantly higher percentages of activated T cells than controls when stained for the activation markers HLA-DR3 and CD69(+) (ACS: P < 0.0001, P = 0.002, CAD: P < 0.0001, P = 0.018, respectively). IL-1, IL-4 and IL-10 proved significantly higher in ACS versus controls (P = 0.036, P = 0.01, P < 0.0001 respectively). Th1 to Th2 ratio shifted towards a Th1 dominance in both diseases. Both general proinflammatory markers and activated T cells signify CAD. The orchestrated proinflammatory cascade eventually leads to the development of the disease.