Effects of 50 Hz magnetic field exposure on the rate of RNA synthesis by normal human fibroblasts.
Effects of 50 Hz magnetic field exposure on the rate of RNA synthesis by normal human fibroblasts.
复制标题
50 Hz 磁场暴露对正常人成纤维细胞 RNA 合成速率的影响。
DOI：
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发表时间：
1996
影响因子：
2.6
通讯作者：
R. Saunders
中科院分区：
文献类型：
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作者：
N. Cridland;N. Sabour;R. Saunders
目的
研究暴露在磁场中是否会影响RNA合成的速率，RNA合成是细胞活性的一个广泛指标。
材料和方法
将正常人成纤维细胞暴露于50 Hz的磁场中，磁场的通量密度范围为2 microT和20 mT。总RNA的合成速率通过[3H]尿苷掺入大分子物质来测定。此外，分离聚腺苷酸化RNA并用于估计mRNA的合成速率。
结果
在所有细胞中，[3H]尿苷掺入总RNA和信使RNA在整个5 h暴露期间逐渐增加。然而，与对照组相比，磁场暴露对总RNA或信使RNA的合成速率没有可检测到的影响。
结论
这些研究结果表明，在检查的条件下，总转录率不受工频磁场暴露的影响。结合以前的数据，这表明，如果磁场确实改变了细胞活动，这种影响可能是极其微妙的。
PURPOSE
To investigate whether exposure to magnetic fields can affect the rate of RNA synthesis, a broad measure of cellular activity.
MATERIALS AND METHODS
Normal human fibroblasts were exposed to 50 Hz magnetic fields at a range of flux densities between 2 microT and 20 mT. The rate of synthesis of total RNA was determined by following the incorporation of [3H]uridine into macromolecular material. In addition, polyadenylated RNA was isolated and used to estimate the rate of synthesis of mRNA.
RESULTS
Incorporation of [3H]uridine into both total and messenger RNA increased progressively throughout the 5 h exposure period in all cells. However, magnetic field exposure had no detectable effect on the rate of synthesis of either total or messenger RNA when compared with controls.
CONCLUSIONS
These findings indicate that under the conditions examined, gross transcription rates are not affected by exposure to power frequency magnetic fields. Taken together with previous data, this suggests that if magnetic fields do alter cellular activity, the effect is likely to be extremely subtle.
影响因子：
56.9
作者：
COHEN, SM;ELLWEIN, LB
通讯作者：
ELLWEIN, LB