Graphene-Oxide-Modified Lanthanide Nanoprobes for Tumor-Targeted Visible/NIR-II Luminescence Imaging
Graphene-Oxide-Modified Lanthanide Nanoprobes for Tumor-Targeted Visible/NIR-II Luminescence Imaging
复制标题
用于肿瘤靶向可见光/NIR-II 发光成像的氧化石墨烯修饰稀土纳米探针
DOI：
10.1002/anie.201909416
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发表时间：
2019-11-08
影响因子：
16.6
通讯作者：
Chen, Xueyuan
中科院分区：
文献类型：
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作者：
Song, Xiaorong;Li, Shihua;Chen, Xueyuan
合成具有用于生物成像的分子识别能力的亲水性镧系元素掺杂纳米晶体（Ln(3+) - 纳米晶体，Ln(3+)-NCs）目前仍是一项挑战。在此，我们提出一种有效的策略来突破这一瓶颈，即将Ln(3+)-NCs包裹在氧化石墨烯（NCs@GO）中。通过优化氧化石墨烯的尺寸以及NaYF4:Yb,Er@NaYF4的核壳结构，制备出了单分散的NCs@GO，从而将纳米晶体强烈的可见光/近红外二区（NIR-II）发光特性与氧化石墨烯独特的表面性质及生物医学功能结合起来。这种纳米结构不仅具有广泛的溶剂分散性、高效的细胞摄取能力和优异的生物相容性，还能够通过简便的步骤用各种试剂（如DNA、蛋白质或纳米颗粒）进行进一步修饰。此外，我们在概念验证实验中证明，NCs@GO能够实现细胞内同时追踪和微小核糖核酸 - 21（microRNA-21）的可视化，以及在1525纳米波长下进行高灵敏度的体内肿瘤靶向NIR-II成像 。
The synthesis of hydrophilic lanthanide-doped nanocrystals (Ln(3+)-NCs) with molecular recognition ability for bioimaging currently remains a challenge. Herein, we present an effective strategy to circumvent this bottleneck by encapsulating Ln(3+)-NCs in graphene oxide (NCs@GO). Monodisperse NCs@GO was prepared by optimizing GO size and core-shell structure of NaYF4:Yb,Er@NaYF4, thus combining the intense visible/near-infrared II (NIR-II) luminescence of NCs and the unique surface properties and biomedical functions of GO. Such nanostructures not only feature broad solvent dispersibility, efficient cell uptake, and excellent biocompatibility but also enable further modifications with various agents such as DNA, proteins, or nanoparticles without tedious procedures. Moreover, we demonstrate in proof-of-concept experiments that NCs@GO can realize simultaneous intracellular tracking and microRNA-21 visualization, as well as highly sensitive in vivo tumor-targeted NIR-II imaging at 1525 nm.