Hprt mutations in human T-lymphocytes reflect radioprotective effects of the aminothiol, WR-1065.
Hprt mutations in human T-lymphocytes reflect radioprotective effects of the aminothiol, WR-1065.
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人类 T 淋巴细胞中的 Hprt 突变反映了氨基硫醇 WR-1065 的辐射防护作用。
DOI：
10.1093/carcin/17.12.2647
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发表时间：
1996
期刊：
影响因子：
4.7
通讯作者：
Nicklas,JA
中科院分区：
文献类型：
--
作者：
Clark,LS;Albertini,RJ;Nicklas,JA
氨基硫醇如WR-2721及其活性游离硫醇WR-1065先前已被证明可以减少指数增长细胞中电离辐射引起的突变。在这项研究中，将未分裂的人t淋巴细胞在外部γ射线照射前30分钟或照射后3小时暴露于氨基硫醇辐射保护剂WR-1065中，随后评估其存活和突变诱导情况。只有当WR-1065存在时，γ辐照引起的细胞毒性才会降低。然而，无论给药时间如何，hprt突变的频率都有所降低，尽管只有在照射前30分钟添加WR-1065时才有统计学意义（P< 0.01）。这是首次利用人类非循环细胞证明WR-1065对辐射诱导的报告基因突变的保护作用。这些细胞体内产生的hprt突变可能有助于监测氨基硫醇在人群中的辐射防护作用。
Aminothiols such as WR-2721 and its active free thiol WR-1065 have previously been shown to reduce mutations resulting from ionizing radiation in exponentially growing cells. In this study, non-dividing human GoT-lymphocytes were exposed to the aminothiol radioprotective agent, WR-1065, 30 min before or 3 h after external beam γ-irradiation and subsequently assessed for survival and mutation induction at thehprtlocus. Cytotoxicity due to γ-irradiation was reduced only when the WR-1065 was present during irradiation. The frequency ofhprtmutations, however, was reduced regardless of time of administration, although the reduction was statistically significant only when WR-1065 was added 30 min before irradiation (P< 0.01). This is the first study to demonstrate the protective effects of WR-1065 against radiation-induced mutation in a reporter gene using a human non-cycling cell.Hprtmutations arisingin vivoin these cells may be useful for monitoring the radioprotective effect of aminothiols in human populations.