DNA barcoding, MALDI-TOF, and AFLP data support Fusarium ficicrescens as a distinct species within the Fusarium fujikuroi species complex
DNA barcoding, MALDI-TOF, and AFLP data support Fusarium ficicrescens as a distinct species within the Fusarium fujikuroi species complex
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DOI：
10.1016/j.funbio.2015.08.001
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发表时间：
2016-02-01
期刊：
影响因子：
2.5
通讯作者：
De Hoog, G. Sybren
中科院分区：
文献类型：
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作者：
Al-Hatmi, Abdullah M. S.;Mirabolfathy, Mansoureh;De Hoog, G. Sybren
Fusarium fujikuroi Fusarium fujikuroi Fusarium species complex （FFSC）是与植物病害、霉菌毒素产生以及创伤性和播散性人类感染相关的最常见镰刀菌群之一。本文报道了一种新分类单元Fusarium ficicrescens sp. nov.的描述和分类，该分类单元采集自伊朗受污染的无花果果实。最初通过形态学鉴定为镰刀菌和diyazi。本研究采用多位点序列分析、扩增片段长度多态性（AFLP）、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和表型特征对该物种进行了研究。基于翻译延伸因子la （TEF1）、RNA聚合酶亚基（RPB2）和β -微管蛋白（BT2）的多位点分析证实了F. ficicrescens是FFSC的成员。系统发育分析表明，该真菌与乳酸镰刀菌、分枝镰刀菌和油状镰刀菌亲缘关系密切；已知的植物病原体，真菌毒素生产者，以及偶尔发生的多重耐药机会主义者。新物种的不同之处在于能够在37摄氏度的环境中生长，并且不产生霉菌毒素。TEF1是鉴定镰刀菌种类的必要条形码。除了TEF1外，我们还评估了BT2和RPB2，以便为新物种的识别提供足够的遗传和物种边界信息。(C) 2015英国真菌学学会。Elsevier Ltd.出版。版权所有。
The Fusarium fujikuroi species complex (FFSC) is one of the most common groups of fusaria associated with plant diseases, mycotoxin production and traumatic and disseminated human infections. Here we present the description and taxonomy of a new taxon, Fusarium ficicrescens sp. nov., collected from contaminated fig fruits in Iran. Initially this species was identified as Fusarium andiyazi by morphology. In the present study the species was studied by multilocus sequence analysis, amplified fragment length polymorphism (AFLP), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and phenotypic characters. Multilocus analyses were based on translation elongation factor la (TEF1), RNA polymerase subunit (RPB2) and beta-tubulin (BT2) and proved F. ficicrescens as a member of the FFSC. Phylogenetic analysis showed that the fungus is closely related to Fusarium lactis, Fusarium ramigenum, and Fusarium napiforme; known plant pathogens, mycotoxin producers, and occasionally occurring multidrug resistant opportunists. The new species differed by being able to grow at 37 degrees C and by the absence of mycotoxin production. TEF1 was confirmed as an essential barcode for identifying Fusarium species. In addition to TEF1, we evaluated BT2 and RPB2 in order to provide sufficient genetic and species boundaries information for recognition of the novel species. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.