Suppressive effect of Clostridium Perfringens-produced heat-stable substance(s) on proliferation of human colon adenocarcinoma HT29 cells in culture
Suppressive effect of Clostridium Perfringens-produced heat-stable substance(s) on proliferation of human colon adenocarcinoma HT29 cells in culture
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DOI：
10.1016/j.canlet.2005.10.016
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发表时间：
2006-09-28
期刊：
影响因子：
9.7
通讯作者：
Ohnishi, Yoshinari
中科院分区：
文献类型：
--
作者：
Arimochi, Hideki;Morita, Kyoji;Ohnishi, Yoshinari
产气荚膜梭菌被认为是增加结肠癌风险的肠道细菌之一。在以前的研究中，我们已经表明，口服C。产气荚膜杆菌培养基可以抑制大鼠结肠中诱变剂诱导的肿瘤前病变的形成，从而表明在该培养基中存在防止结肠肿瘤发生的因子。然而，这种抑制作用的有效因子的性质和机制仍有待研究。然后，研究了C.检查了产气荚膜杆菌培养基对人结肠腺癌HT 29细胞的作用。HT 29细胞暴露于C.发现产气荚膜杆菌培养基抑制这些细胞的增殖，这可能是通过减少立即早期基因IFN-1表达。这些观察结果表明C.产气荚膜杆菌培养基对结肠肿瘤细胞具有细胞抑制作用，这可能是预防大鼠结肠中肿瘤前形成的原因。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Clostridium perfringens has been regarded as one of the intestinal bacteria increasing colon cancer risk. In previous studies, we have shown that the oral administration of C. perfringens culture medium can inhibit the mutagen-induced formation of pre-neoplastic lesions in rat colon, thus proposing the existence of factor(s) preventing colon tumorigenesis in this culture medium. However, the properties of effective factor(s) and the mechanism of this inhibitory action still remain to be investigated. Then, the effect of C. perfringens culture medium on human colon adenocarcinoma HT29 cells was examined. The exposure of HT29 cells to C. perfringens culture medium was found to suppress the proliferation of these cells probably through the reduction of immediate early gene egr-1 expression. These observations suggest that C. perfringens culture medium has a cytostatic action on colon tumor cells, which may be responsible for the prevention of pre-neoplastic formation in rat colon. (c) 2006 Elsevier Ireland Ltd. All rights reserved.