Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonization factor detected in human enterotoxigenic Escherichia coli
Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonization factor detected in human enterotoxigenic Escherichia coli
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猪和牛血清流行病学调查人产肠毒素大肠杆菌中新型K88（F4）样定植因子
DOI：
10.1101/2021.05.01.442292
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Yoshikazu Nishikawa
中科院分区：
文献类型：
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作者：
Yoshihiko Tanimoto;Miyoko Inoue;Kana Komatsu;Atsuyuki Odani;Takayuki Wada;Eriko Kage-Nakadai;Yoshikazu Nishikawa
产肠毒素大肠杆菌（ETEC）菌株表达各种菌毛或非菌毛定植因子（CF）和肠毒素是肠道疾病的关键原因。人ETEC血清型O 169：H41（O 169）是全球流行性ETEC的代表;该微生物显示出与肠聚集性E.杆菌先前，我们确定了不稳定毒力质粒pEntYN 10的完整序列。该质粒包括一组独特的基因，编码一种类似于猪ETEC K88（F4）的新型CF，以及CS6（一种众所周知的人ETEC代表性CF）和另一种类似于人ETEC CS 8（CFA/III）的新型CF。在本研究中，我们重点关注K88样CF（以下简称K88 O 169），它可能允许微生物感染家畜，就像原始的K88携带菌株一样，可导致仔猪腹泻。检测样本中是否存在针对可能的旁系同源粘附素重组蛋白（来自K88 O 169的FaeG 1和FaeG 2）以及典型K88（F4）的FaeG的抗体。使用重组抗原（来自K88 O 169的两种旁系同源物FaeG 1和FaeG 2）进行的血清流行病学研究显示，在间接ELISA试验中，猪（18.0%）和牛（17.1%）血清对K88 O 169 FaeG 1和/或FaeG 2抗原具有反应性。这些结果表明，E.携带K88 O 169粘附素的大肠杆菌可感染猪、牛等多种宿主。这是国内首次报道家畜具有抗人ETEC K88 O 169抗体。尽管基于CF，认为人ETEC与家畜ETEC不同，但人畜共患病菌株可能隐藏在人ETEC微生物中。应采用“一个健康”的概念来干预动物和人类的ETEC感染。
Enterotoxigenic Escherichia coli (ETEC) strains that express various fimbrial or nonfimbrial colonisation factors (CFs) and enterotoxins are critical causes of diarrhoeal diseases. Human ETEC serotype O169:H41 (O169) has been a representative of epidemic ETEC worldwide; the organism shows massive adherence to HEp-2 cells similar to enteroaggregative E. coli. Previously, we determined the complete sequence of the unstable virulence plasmid, pEntYN10. The plasmid included a unique set of genes encoding a novel CF resembling K88 (F4) of porcine ETEC, in addition to CS6, a well-known representative CF of human ETEC, and another novel CF similar to CS8 (CFA/III) of human ETEC. In the present study, we focused on K88-like CF (hereafter, K88O169) that may allow the organisms to infect domestic livestock like original K88-harbouring strains that can cause diarrhoea in piglets. Samples were tested for antibodies against recombinant proteins of possible paralogous adhesins, FaeG1 and FaeG2, from K88O169 and the FaeG of typical K88 (F4). The seroepidemiological study using recombinant antigens (two paralogs FaeG1 and FaeG2 from K88O169) showed reactivity of porcine (18.0%) and bovine (17.1%) sera to K88O169 FaeG1 and/or FaeG2 antigens on indirect ELISA tests. These results suggest that E. coli with K88O169 adhesin can infect various hosts, including pigs and cattle. This is the first report of domestic livestock having antibodies to K88O169 of human ETEC. Although human ETEC had been thought to be distinguished from those of domestic animals based on CFs, zoonotic strains may conceal themselves among human ETEC organisms. The concept of One Health should be adopted to intervene in ETEC infections among animals and humans.