THE 30-KILODALTON GENE-PRODUCT OF TOBACCO MOSAIC-VIRUS POTENTIATES VIRUS MOVEMENT
THE 30-KILODALTON GENE-PRODUCT OF TOBACCO MOSAIC-VIRUS POTENTIATES VIRUS MOVEMENT
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DOI：
10.1126/science.237.4813.389
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发表时间：
1987-07-24
期刊：
影响因子：
56.9
通讯作者：
BEACHY, RN
中科院分区：
文献类型：
--
作者：
DEOM, CM;OLIVER, MJ;BEACHY, RN
烟草花叶病毒的30千道尔顿（kD）蛋白的作用是在感染期间促进病毒的细胞间传播。为了直接确定该蛋白质的功能，通过Ti质粒介导的根癌农杆菌转化系统，将含有30-kD蛋白质基因的克隆互补DNA的嵌合基因导入烟草细胞。从转化烟草细胞再生的转基因植株表达30-kD蛋白信使RNA并积累30-kD蛋白。表达30 kD蛋白基因的幼苗补充了TMV的Ls 1突变体，该突变体在细胞间运动中对温度敏感。此外，在表达30 kD蛋白基因的幼苗中检测到Ls 1病毒在允许温度下的增强运动。这些结果最终证明，烟草花叶病毒的30 kD蛋白增强了病毒在细胞之间的运动。
The proposed role of the 30-kilodalton (kD) protein of tobacco mosaic virus is to facilitate cell-to-cell spread of the virus during infection. To directly define the function of the protein, a chimeric gene containing a cloned complementary DNA of the 30-kD protein gene was introduced into tobacco cells via a Ti plasmid-mediated transformation system ofAgrobacterium tumefaciens. Transgenic plants regenerated from transformed tobacco cells expressed the 30-kD protein messenger RNA and accumulated 30-kD protein. Seedlings expressing the 30-kD protein gene complemented the Ls1 mutant of TMV, a mutant that is temperature-sensitive in cell-to-cell movement. In addition, enhanced movement of the Ls1 virus at the permissive temperature was detected in seedlings that express the 30-kD protein gene. These results conclusively demonstrate that the 30-kD protein of tobacco mosaic virus potentiates the movement of the virus from cell to cell.