Calmodulin-binding proteins of bovine thyroid plasma membranes.
Calmodulin-binding proteins of bovine thyroid plasma membranes.
复制标题
牛甲状腺质膜的钙调蛋白结合蛋白。
DOI：
10.1016/0304-4165(82)90116-7
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Field,JB
中科院分区：
文献类型：
--
作者：
Tawata,M;Field,JB
用125I标记的钙调蛋白凝胶覆盖技术研究了牛甲状腺质膜上的钙调蛋白结合蛋白。纯化的甲状腺质膜含有两种钙调素结合蛋白，其相对分子质量约为2。分别为22万和15万。过量的未标记钙调素、100μM三氟拉嗪或1 mMEGTA抑制了125I标记的钙调素与钙调素结合蛋白的结合，表明这种结合是钙调素专一性的和钙依赖的。钙调蛋白结合蛋白似乎是细胞骨架的组成部分，因为它们在用1%Triton X-100处理甲状腺质膜后仍留在颗粒中。类似的钙调蛋白结合蛋白存在于大鼠的肝脏质膜中，但不存在于人的红细胞质膜中。这两种钙调蛋白结合蛋白可能与细胞骨架的其他成分相互作用，调节甲状腺细胞的内吞、胞吐和激素分泌。
Calmodulin binding proteins in bovine thyroid plasma membranes were investigated using the125I-labeled calmodulin gel overlay technique. The purified thyroid plasma membranes contained two calmodulin binding proteins with molecular weights of approx. 220 000 and 150 000 respectively. The binding of125I-labeled calmodulin to the calmodulin binding proteins was inhibited by excess unlabeled calmodulin, 100 μM trifluoperazine or 1 mM EGTA, indicating that the binding was calmodulin-specific and calcium-dependent. The calmodulin binding proteins appear to be components of the cytoskeleton since they remained in the pellet after treatment of the thyroid plasma membranes with 1% Triton X-100. Similar calmodulin binding proteins were present in rat liver plasma membranes, but not in human red blood cell plasma membranes. These two calmodulin binding proteins may interact with other components of the cytoskeleton and regulate endocytosis, exocytosis and hormone secretion in thyroid cells.