TRANSFER OF C-4 PHOTOSYNTHETIC CHARACTERS THROUGH HYBRIDIZATION OF FLAVERIA SPECIES
TRANSFER OF C-4 PHOTOSYNTHETIC CHARACTERS THROUGH HYBRIDIZATION OF FLAVERIA SPECIES
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DOI：
10.1104/pp.90.4.1538
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发表时间：
1989-08-01
期刊：
影响因子：
7.4
通讯作者：
BROWN, RH
中科院分区：
文献类型：
--
作者：
CAMERON, RG;BASSETT, CL;BROWN, RH
通过黄花菊（Flaveria trinervia，春）杂交，研究了C4光合性状的转移。Mohr （C4）与黄草鲍威尔（c4样）与黄草属线性滞后。（C3- c4）和C3种黄花菊（C3）。除褐飞虱属外，其育性较低，主要是由于染色体配对不规则和花粉染色率低。二价配对，花粉可染率76%。杂交种的表观光合作用值为中亲本平均值的71 ~ 148%，而CO2补偿浓度与C4或类C4亲本相似，但以C3种品蕾为亲本的杂交种除外。O2对表观光合作用的抑制、磷酸烯醇丙酮酸羧化酶和nadp -苹果酸酶的活性和亚基水平接近于C3或C3- c4亲本。褐螺旋藻和三角花螺旋藻在将AP的O2抑制值和CO2补偿浓度值传递给线性螺旋藻（C3-C4）的能力相同，但三角花螺旋藻的PEPC活性更高。O2对AP的抑制作用与磷酸烯醇丙酮酸羧化酶（r = -0.95）和nadp -苹果酸酶（r = -0.87）活性的对数相关。这些结果证实C4性状可以通过C3-C4和C4或C4样种的杂交转移，C4的光合作用程度高于C3-C4种，至少在褐飞虱中是如此。F.线性，可育的后代。
Transfer of C4 photosynthetic traits was studied through hybridization of Flaveria trinervia (Spreng.) Mohr (C4) and Flaveria brownii A.M. Powell (C4-like) with Flaveria linearis Lag. (C3-C4) and the C3 species Flaveria pringlei Gandoger (C3). Fertility was low, based on irregular chromosome pairing and low pollen stainability, except in F. brownii .times. F. linearis which had bivalent pairing and 76% stainable pollen. Hybrids had apparent photosynthesis values of 71 to 148% of the midparental means, while the CO2 compensation concentration was similar to the C4 or C4-like parent, except in hybrids having the C3 species F. pringlei as a parent. Inhibition of apparent photosynthesis by O2, and phosphoenolpyruvate carboxylase and NADP-malic enzyme activities and subunit levels in the hybrids were closer to the C3 or C3-C4 parent. The species F. brownii and F. trinervia were equal in their capacity to transfer reduced O2 inhibition of AP and CO2 compensation concentration values to hybrids with F. linearis (C3-C4), although hybrids with F. trinervia had higher PEPC activity. The O2 inhibition of AP was correlated with the logarithm of activities of phosphoenolpyruvate carboxylase (r = -0.95) an NADP-malic enzyme (r = -0.87). These results confirm that C4 traits can be transferred by hybridization of C3-C4 and C4 or C4-like species, with a higher degree of C4 photosynthesis than exists in C3-C4 species, and at least in F. brownii .times. F. linearis, fertile progeny are obtained.