Upregulation of surfactant protein a in chronic rhinosinusitis
Upregulation of surfactant protein a in chronic rhinosinusitis
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DOI：
10.1097/01.mlg.0000194223.22763.5f
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发表时间：
2006-02-01
期刊：
影响因子：
2.6
通讯作者：
Hwang, SJ
中科院分区：
文献类型：
--
作者：
Lee, HM;Kang, HJ;Hwang, SJ
目标.表面活性蛋白A（SurfactantProteinA，SP-A）是一种在哺乳动物的第一线宿主防御中起重要作用的蛋白质。然而，SP-A在人类鼻窦粘膜中的存在并不为人所知。本研究旨在探讨SP-A蛋白在人副鼻窦粘膜中的表达，并比较正常副鼻窦粘膜和慢性鼻窦炎副鼻窦粘膜中SP-A mRNA的表达。方法.取10例慢性鼻窦炎无息肉手术患者和10例正常对照者的副鼻窦粘膜标本。逆转录聚合酶链反应检测SP-A mRNA。用密度计半定量SP-A转录本的表达水平。用免疫组化方法观察SP-A的细胞定位。结果：人鼻窦粘膜中有SP-A mRNA和蛋白表达。慢性鼻-鼻窦炎鼻窦黏膜中SP-A/GAPDHmRNA比例高于正常鼻窦黏膜（P < .05）。免疫组化染色显示，在对照组和慢性鼻窦炎患者的鼻窦粘膜上皮细胞和粘膜下腺体的SP-A免疫反应。慢性鼻窦炎粘膜免疫反应性强于正常鼻窦粘膜。结论：正常人和病变人鼻窦粘膜均存在SP-A mRNA和蛋白。这些结果可能为慢性鼻窦炎的治疗提供新的靶点。
Objectives. Surfactant protein A (SP-A) is protein that appears to play an important role in mammalian first-line host defense. However, the presence of SP-A in the human paranasal sinus mucosa is not well known. The purpose of this study was to investigate the expression of SP-A protein in human paranasal sinus mucosa and to compare the expression of SP-A mRNA between normal paranasal sinus mucosa and paranasal sinus mucosa with chronic rhinosinusitis. Methods. Paranasal sinus mucosa samples from 10 patients who underwent surgery for chronic rhinosinusitis without polyps and 10 normal control subjects were used. Reverse transcriptase polymerase chain reaction was done to detect SP-A mRNA. The expression level of SP-A transcripts was semiquantified with desitometry. Cellular localization of SP-A was sought by using immunohistochemistry. Results: SP-A mRNA and protein were expressed in the human paranasal sinus mucosa. SP-A/GAPDH mRNA ratio in the paranasal sinus mucosa with chronic rhinosinusitis was greater compared with that in normal paranasal sinus mucosa (P < .05). Immunohistochemical staining revealed SP-A immunoreactivity in the epithelial cells and submucosal glands of paranasal sinus mucosa in both control subjects and chronic sinusitis patients. Stronger immunoreactivity was observed in chronic rhinosinusitis mucosa as compared with normal paranasal sinus mucosa. Conclusion: SP-A mRNA and protein are present in both normal and diseased human paranasal sinus mucosa. These results may provide potential targets for novel therapy of chronic rhinosinusitis.