Protein partitioning and transport in supported cationic acrylamide-based hydrogels
Protein partitioning and transport in supported cationic acrylamide-based hydrogels
复制标题
支持的阳离子丙烯酰胺基水凝胶中的蛋白质分配和运输
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
G. Carta
中科院分区：
文献类型：
--
作者：
S. Russell;E. B. Belcher;G. Carta
肌红蛋白在阳离子丙烯酰胺基水凝胶中的分配和传输研究了显微镜可视化方法。均匀的阳离子凝胶内合成的熔融石英毛细管与方形截面，这允许在瞬态吸附和解吸过程中的蛋白质浓度分布的直接测定。观察到扩散的自相似曲线，并用于确定凝胶中的平衡蛋白质结合能力和蛋白质扩散率。传质速率被发现是基本上独立的外部溶液浓度，但变化显着的凝胶聚合物浓度。基于吸附相浓度梯度的通量的Fickian扩散模型与正和负蛋白质浓度步骤的实验确定的浓度曲线是一致的。毛细管支撑的凝胶的平衡和速率参数确定也比较有利的宏观测量，使用复合离子交换介质，包括类似的凝胶多孔二氧化硅颗粒的孔内举行的那些获得。
The partitioning and transport of myoglobin in cationic, acrylamide-based hydrogels are studied by a microscopic visualization method. Homogeneous cationic gels are synthesized inside fused-silica capillaries with a square section, which allow a direct determination of protein concentration profiles during transient adsorption and desorption. Diffuse, self-similar profiles are observed and used to determine the equilibrium protein binding capacity and the protein diffusivity in the gel. Mass-transfer rates are found to be essentially independent of the external solution concentration, but to vary dramatically with the gel polymer concentration. A Fickian diffusion model with a flux based on the adsorbed-phase concentration gradient is consistent with the experimentally determined concentration profiles for both positive and negative protein concentration steps. The equilibrium and rate parameters determined for the capillary-supported gels also compare favorably with those obtained from macroscopic measurements using composite ion-exchange media comprising similar gels held within the pores of porous silica particles.
使用正压输注在大脑中分布大分子染料：治疗剂直接控制输送的模型。
影响因子：
--
作者：
Prabhu,SS;Broaddus,WC;Gillies,GT;Loudon,WG;Chen,ZJ;Smith,B
通讯作者：
Smith,B