Modulation of Microtubule Dynamics by the Microtubule-Associated Protein 1a
Modulation of Microtubule Dynamics by the Microtubule-Associated Protein 1a
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DOI：
10.1002/jnr.21920
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发表时间：
2009-04-01
影响因子：
4.2
通讯作者：
Brown, David L.
中科院分区：
文献类型：
--
作者：
Faller, Elliott M.;Brown, David L.
结构微管相关蛋白（MAP）与微管相互作用，调节微管的各个动态阶段。本研究的目的是测量 myc 标记的 MAP1a 片段对体内微管动态相的影响。来自已用人绿色荧光蛋白 (GFP)-α-微管蛋白永久转染的上皮肾细胞系 (LLCPK1) 的细胞被 myc 标记的 MAP1a 片段瞬时转染。表达 MAP1a 片段的细胞用于使用荧光显微镜直接观察活细胞中的微管动态。所有包含微管结合域的截短 MAP1a 重链片段均显示与微管相关。含有 MAP1a 投影结构域不同区域的截短片段通过促进生长或抑制缩短阶段，表现出对微管动态事件的影响存在差异。与 MAP1a 相比，MAP2c 捆绑微管并导致微管运动完全停止。本研究的结果表明 MAP1a 促进微管缓慢、稳定的生长。这种类型的生长对于成年神经元的维持和重组可能很重要。 (C) 2008 Wiley-Liss, Inc.
Structural microtubule-associated proteins (MAPs) interact with microtubules to regulate the various dynamic stages of microtubules. The purpose of this study was to measure the impact of myc-tagged MAP1a fragments on microtubule dynamic phases in vivo. Cells from an epithelial kidney cell line (LLCPK1) that had been permanently transfected with human green fluorescent protein (GFP)-alpha-tubulin were transiently transfected with myc-tagged MAP1a fragments. Cells expressing MAP1a fragments were used to make direct observations of microtubule dynamics in living cells using fluorescence microscopy. All truncated MAP1a heavy chain fragments that contained the microtubule-binding domain were shown to associate with microtubules. Truncated fragments containing different regions of the projection domain of MAP1a demonstrated variations in their impact on microtubule dynamic events by promoting growth or inhibition of shortening phases. In contrast to MAP1a, MAP2c bundled microtubules and resulted in a complete arrest of microtubule motility. Results from the present study suggest that MAP1a promotes slow, stable growth of microtubules. This type of growth may be important in the maintenance and restructuring of adult neurons. (C) 2008 Wiley-Liss, Inc.