The Involvement of the Oxidative Stress in Murine Blue LED Light-Induced Retinal Damage Model
The Involvement of the Oxidative Stress in Murine Blue LED Light-Induced Retinal Damage Model
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DOI：
10.1248/bpb.b16-01008
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发表时间：
2017-08-01
影响因子：
2
通讯作者：
Hara, Hideaki
中科院分区：
文献类型：
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作者：
Nakamura, Maho;Kuse, Yoshiki;Hara, Hideaki
本研究旨在建立蓝光二极管(LED)光诱导的小鼠视网膜损伤模型，并评价抗氧化剂N-乙酰半胱氨酸(NAC)的作用。将小鼠暴露于400或800 lx蓝光下2 h，5 d后以视网膜电波波幅和外核层厚度评价视网膜损伤。此外，我们还通过免疫组织化学方法研究了蓝光照射对短波敏感视蛋白(S-视蛋白)和视紫红质表达的影响。蓝光诱导视网膜光损伤，导致S视蛋白崩解，视紫质由内、外节段定位改变至ONL。相反，在暗适应前和光照前，NAC以100或250 mg/kg的剂量每天两次腹腔注射。NAC对蓝光诱导的视网膜损伤有保护作用，且呈剂量依赖关系。此外，蓝光诱导的视紫红质定位改变和S-视蛋白水平的降低被NAC抑制。我们建立了蓝光诱导视网膜损伤的小鼠模型，这些发现表明氧化应激部分参与了蓝光诱导的视网膜损伤。
The aim of study was to establish a mouse model of blue light emitting diode (LED) light-induced retinal damage and to evaluate the effects of the antioxidant N-acetylcysteine (NAC). Mice were exposed to 400 or 800 lx blue LED light for 2 h, and were evaluated for retinal damage 5d later by electroretinogram amplitude and outer nuclear layer (ONL) thickness. Additionally, we investigated the effect of blue LED light exposure on shorts-wave-sensitive opsin (S-opsin), and rhodopsin expression by immunohistochemistry. Blue LED light induced light intensity dependent retinal damage and led to collapse of S-opsin and altered rhodopsin localization from inner and outer segments to ONL. Conversely, NAC administered at 100 or 250 mg/kg intraperitoneally twice a day, before dark adaptation and before light exposure. NAC protected the blue LED light-induced retinal damage in a dose-dependent manner. Further, blue LED light-induced decreasing of S-opsin levels and altered rhodopsin localization, which were suppressed by NAC. We established a mouse model of blue LED light-induced retinal damage and these findings indicated that oxidative stress was partially involved in blue LED light-induced retinal damage.