The association of biologically active lipids with the development of transfusion-related acute lung injury: A retrospective study
The association of biologically active lipids with the development of transfusion-related acute lung injury: A retrospective study
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DOI：
10.1046/j.1537-2995.1997.37797369448.x
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发表时间：
1997-07-01
期刊：
影响因子：
2.9
通讯作者：
Ambruso, DR
中科院分区：
文献类型：
--
作者：
Silliman, CC;Paterson, AJ;Ambruso, DR
背景：输血相关急性肺损伤（TRALI）在临床上与成人呼吸窘迫综合征（ARDS）相似，并且与输注血液成分中的白细胞抗体有关。动物模型表明 ARDS 与中性粒细胞 (PMN) 启动剂有关；然而，需要两名特工。先前的研究表明，血液储存过程中会产生中性粒细胞引发剂。因此，检查了 PMN 引发剂与 TRALI 的关联。 研究设计和方法：对 10 名伴有发热或荨麻疹反应的 TRALI 和 IO 患者（对照组）进行了评估。通过将中性粒细胞与患者的输血前和输血后血液样本一起孵育，然后激活呼吸爆发，测试了中性粒细胞启动活性的存在。通过正相高效液相色谱（HPLC）分离血浆脂质，并评估引发活性。在献血者和 TRALI 患者中测定了白细胞抗体的存在。结果：TRALI 患者的输血后血清（11.4 +/- 1.8 nmol 超氧阴离子/分钟，平均 rt SEM；n = 10）和血浆中存在显着更多的 PMN 引发活性，比输血前血清（6.5 +/- 1.5；n = 10）或输血前和输血后血清（5.1 +/- 1.3，n = 10；和 4.5 +/- 1.4，分别为 n = 10）和对照（p
BACKGROUND: Transfusion-related acute lung injury (TRALI) is clinically similar to the adult respiratory distress syndrome (ARDS) and has been linked to the transfusion of leukocyte antibodies in blood components. Animal models have implicated neutrophil (PMN)-priming agents in ARDS; however, two agents were required. Previous studies showed the generation of PMN-priming agents during blood storage. Thus, the association of PMN-priming agents with TRALI was examined.STUDY DESIGN AND METHODS: Ten patients with TRALI and IO with febrile or urticarial reactions (control group) were evaluated. The presence of PMN-priming activity was tested in the patients' pretransfusion and posttransfusion blood samples by incubating PMNs with these samples followed by activation of the respiratory burst. Plasma lipids were separated by normal-phase high-performance liquid chromatography (HPLC), and the priming activity was evaluated. The presence of leukocyte antibodies was determined in the blood donors and patients with TRALI. RESULTS: Significantly more PMN-priming activity was present in the posttransfusion sera (11.4 +/- 1.8 nmol superoxide anion/min, mean rt SEM; n = 10) and plasma of patients with TRALI than in their pretransfusion sera (6.5 +/- 1.5; n = 10) or in the pretransfusion and posttransfusion sera (5.1 +/- 1.3, n = 10; and 4.5 +/- 1.4, n = 10, respectively) and from the controls (p