RAPID ISOLATION OF HIGH MOLECULAR-WEIGHT PLANT DNA
RAPID ISOLATION OF HIGH MOLECULAR-WEIGHT PLANT DNA
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DOI：
10.1093/nar/8.19.4321
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
THOMPSON, WF
中科院分区：
文献类型：
--
作者：
MURRAY, MG;THOMPSON, WF
本发明提供了一种快速分离高分子量植物DNA（长度为50，000个碱基对或更长）的方法，该DNA不含干扰限制性内切酶完全消化的污染物。该程序产生总细胞DNA（即核、叶绿体和线粒体DNA）。该技术对于从许多不同物种中快速分离少量DNA是理想的，并且对于大规模分离也是有用的。
A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.