Comparison of arsenate reduction and release by three As(V)-reducing bacteria isolated from arsenic-contaminated soil of Inner Mongolia, China
Comparison of arsenate reduction and release by three As(V)-reducing bacteria isolated from arsenic-contaminated soil of Inner Mongolia, China
复制标题
内蒙古砷污染土壤中三种As(V)还原菌还原和释放砷的比较
DOI：
10.1016/j.chemosphere.2016.06.102
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发表时间：
2016
期刊：
影响因子：
8.8
通讯作者：
Cui Yanshan
中科院分区：
文献类型：
--
作者：
Cai Xiaolin;Zhang Zhennan;Yin Naiyi;Du Huili;Li Zejiao;Cui Yanshan
砷（As）污染已成为世界性的环境问题，尤其是砷（As（Ⅲ））已对人类健康构成重大威胁。本文报道了从中国内蒙古赤峰市土壤中分离到的三株厌氧As（Ⅴ）还原菌（菌株JQ、DJ-3和DJ-4）。菌株JQ、DJ-3和DJ-4分别与芽孢杆菌属、脱硫菌属和硫杆菌属亲缘关系较近。其中JQ和DJ-3具有arsC基因，DJ-4具有arsA基因。在缺氧条件下，3株菌均能抵抗和还原高浓度的As（Ⅴ）。对As（Ⅴ）的抗性顺序为DJ-3 > JQ > DJ-4。菌株DJ-3不仅对As（Ⅴ）具有最强的抗性，而且在60 mM As（Ⅴ）处理5 d后，可将53%的As（Ⅴ）还原为As（Ⅲ）。3株菌均能从针铁矿中释放砷，其中DJ-4菌株对针铁矿中砷的释放率最高，为90.5%。这些结果表明，菌株JQ、DJ-3和DJ-4可能在土壤中As的活化和转化中发挥重要作用。
Arsenic (As) contamination has become a worldwide environmental problem: arsenite (As(Ⅲ)) especially has posed a major threat to human health. Here, we report the first three isolates of anaerobic As(Ⅴ)-reducing bacterial strains (strains JQ, DJ-3 and DJ-4) from a soil sample containing 48.7% of total As in the form of As(III) collected in Chifeng, Inner Mongolia, China. Strains JQ, DJ-3 and DJ-4 were phylogenetically closely related toBacillus,DesulfitobacteriumandExiguobacterium, respectively. Among these strains, JQ and DJ-3 have thearsCgene, DJ-4 possesses thearrAgene. The three strains could all resist and reduce high concentrations of As(Ⅴ) under anoxic conditions. The order of resistance to As(Ⅴ) was DJ-3 > JQ > DJ-4. Strain DJ-3 not only possesses the strongest resistance to As(Ⅴ) but could also reduce 53% of the As(Ⅴ) to As(III) in the treatment of 60 mM As(Ⅴ) in 5 d. All three strains could release As from goethite; strain DJ-4 has the highest ability to promote the release of As (90.5%) from goethite. These results suggested that strains JQ, DJ-3 and DJ-4 may play an important role in the mobilization and transformation of As in soil.