FIBRONECTIN IN THE MICROVASCULATURE - LOCALIZATION IN THE PERICYTE ENDOTHELIAL INTERSTITIUM
FIBRONECTIN IN THE MICROVASCULATURE - LOCALIZATION IN THE PERICYTE ENDOTHELIAL INTERSTITIUM
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DOI：
10.1016/s0022-5320(83)90133-8
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
BOYLES, J
中科院分区：
文献类型：
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作者：
COURTOY, PJ;BOYLES, J
[大鼠]毛细血管的周细胞是一种有趣的细胞，在形态和行为的某些方面与大血管的平滑肌细胞相似。本文详细探讨了它们与下层内皮的关系。在新鲜和固定组织上使用间接荧光免疫细胞化学技术，发现纤维连接蛋白（许多组织培养系统中的一种粘附蛋白）集中在血管周围的斑点上，仅在完全灌注的制剂的基底膜上隐约可见。通过电镜，使用过氧化物酶免疫细胞化学标记物，这些纤维连接蛋白的浓度定位于周细胞内皮间质。采用新固定方法的透射电镜检查显示沿周细胞膜排列的微丝和致密斑块，间隙内有纤维和基底膜样物质。这些元素的排列表明两个细胞之间存在机械联系。这种联系将允许周细胞的收缩或松弛影响血管直径。
The pericytes of [rat] capillaries are interesting cells which resemble the smooth muscle cells of larger vessels in some aspects of their morphology and behavior. Here, their relationship to the underlying endothelium was investigated in some detail. Using indirect, fluorescent immunocytochemical techniques on fresh and fixed tissues, it was found that fibronectin (an adhesive protein in many tissue culture systems) is concentrated in spots along vessels and is only faintly visible in the basement membranes of exhaustively perfused preparations. By EM, using a peroxidase immunocytochemical marker, these concentrations of fibronectin were localized to the pericyte-endothelial interstitia. Examination by TEM [transmission electron microscopy] using a new fixation procedure demonstrated the organization of microfilaments and dense plaques along the pericyte membrane with fibrous and basement membrane-like material within this interstitial space. The arrangements of these elements suggest a mechanical linkage between the 2 cells. Such a linkage would allow contractions or relaxation of the pericyte to affect vessel diameter.