Pedobacter aquatilis sp nov., isolated from drinking water, and emended description of the genus Pedobacter
Pedobacter aquatilis sp nov., isolated from drinking water, and emended description of the genus Pedobacter
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DOI：
10.1099/ijs.0.64176-0
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发表时间：
2006-08-01
影响因子：
2.8
通讯作者：
Ventosa, Antonio
中科院分区：
文献类型：
--
作者：
Gallego, Virginia;Garcia, Maria Teresa;Ventosa, Antonio
从塞维利亚（西班牙）的饮用水分配系统中分离到一种革兰氏阴性、杆状、不形成孢子的细菌（菌株AR 107（T））。一个多相分类学研究的分离物导致其鉴定为一个成员属土壤杆菌。根据16 S rRNA基因序列比较，菌株AR 107（T）属于拟杆菌门（Bacteroidetes），与土壤杆菌属（Pedobacter）的成员相关。显示95（.）2%的序列相似性，94（.）1%的相似性。主要脂肪酸为异-C-15：0、异-C-17：0 3-OH和总特征3（异-C-15：0 2-OH和/或C-16：1 omega 7 c），这支持菌株AR 107（T）与土壤杆菌属的联系。该菌株的DNA G + C含量为38mol%。根据表型、系统发育和基因型结果，菌株AR 107（T）代表一个新种，建议将其命名为水生足杆菌（Pedobacter aquatilis sp. nov.）。模式菌株为AR 107（T）（=CCM 7347（T）= CECT 7114（T）=JCM 13454（T））。
A Gram-negative, rod-shaped, non-spore-forming bacterium (strain AR107(T)) was isolated from the drinking water distribution system of Seville (Spain). A polyphasic taxonomic study of the isolate resulted in its identification as a member of the genus Pedobacter. On the basis of 16S rRNA gene sequence comparisons, strain AR107(T) was shown to belong to the phylum Bacteroidetes, being related to members of the genus Pedobacter. It showed 95(.)2% sequence similarity with respect to the type strains of Pedobacter heparinus and Pedobacter piscium, and 94(.)1 % similarity with respect to the type strain of Pedobacter himalayensis. The predominant fatty acids were iso-C-15: 0, iso-C-17:0 3-OH and summed feature 3 (iso-C-15:0 2-OH and/or C-16:1 omega 7c), which supports the affiliation of strain AR107(T) to the genus Pedobacter. The DNA G + C content of this strain was 38 mol%. On the basis of the phenotypic, phylogenetic and genotypic results, strain AR107(T) represents a novel species, for which the name Pedobacter aquatilis sp. nov. is proposed. The type strain is AR107(T) (=CCM 7347(T)= CECT 7114(T)=JCM 13454(T)).