Biostimulants enhance growth and drought tolerance in Arabidopsis thaliana and exhibit chemical priming action
Biostimulants enhance growth and drought tolerance in Arabidopsis thaliana and exhibit chemical priming action
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DOI：
10.1111/aab.12482
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发表时间：
2019-03-01
影响因子：
2.6
通讯作者：
Liu, Fuquan
中科院分区：
文献类型：
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作者：
Fleming, Thomas R.;Fleming, Colin C.;Liu, Fuquan
近年来，生物刺激素在农业中的使用一直在稳步增长，其好处已被种植者认识到。来自工业界日益增长的兴趣导致了市场上产品数量的激增，其中许多产品的来源多种多样，如微生物、植物提取物、水解氨基酸和藻类提取物。然而，科学界对生物刺激素领域的认识一直较慢。这是由于对许多生物刺激素产品的作用方式的基础研究有限，以及新的多化合物产品进入市场的速度。在这项研究中，我们利用模式植物拟南芥（Arabidopsis thaliana）开发了一种易于重复的生物测定方法，以测试干旱条件下生物刺激素的功效并评估任何化学启动作用。我们筛选了三种具有生物刺激素作用的产品，它们分别来自氨基酸（Delfan Plus）、藤蔓提取物（Phylgreen）或亚磷酸钾（Trafos K）。在土壤逐渐干旱的条件下，我们测量了植物生长、生化含量和基因表达水平的变化。我们的研究结果证明了生物刺激素介导的抗旱性，产品需要不同的施用时间才能成功缓解压力。生物化学和基因表达变化分析为植物在干旱胁迫前进行生物刺激剂预处理提供了化学启动作用的证据。
The usage of biostimulants in agriculture has been steadily increasing in recent years, and their benefits have been recognised by growers. The growing interest from industry has led to a boom in the number of products on the market, many of which are derived from a diverse range of sources such as microbials, plant extracts, hydrolysed amino acids and algal extracts. However, there has been a slower recognition of the biostimulant sector by the scientific community. This has been a result of limited fundamental research into the modes of action of many biostimulant products and the speed at which new multi-compound products have entered the market. In this study, we have developed a readily reproducible bioassay using the model plant Arabidopsis thaliana to test biostimulant efficacy under drought conditions and assess any chemical priming action. We have screened three products with biostimulant action derived from amino acids (Delfan Plus), Ascophyllum nodosum extract (Phylgreen) or potassium phosphite (Trafos K). Under a progressive soil drought condition, we measured changes in plant growth, biochemical content and gene expression levels. Our results demonstrated biostimulant-mediated drought tolerance, with the products requiring different application timings for successful stress mitigation. The analysis of the biochemical and gene expression changes provided evidence of chemical priming action when plants were pre-treated with biostimulants prior to the drought stress exposure.