Human papillomavirus infection is associated with decreased levels of GM-CSF in cervico-vaginal fluid of infected women
Human papillomavirus infection is associated with decreased levels of GM-CSF in cervico-vaginal fluid of infected women
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DOI：
10.1016/j.jcv.2013.07.001
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发表时间：
2013-10-01
影响因子：
8.8
通讯作者：
Zauli, Giorgio
中科院分区：
文献类型：
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作者：
Comar, Manola;Monasta, Lorenzo;Zauli, Giorgio
背景：虽然人乳头瘤病毒(HPV)是最常见的性传播感染，但关于该病毒对体外受精结局的影响的数据很少。目的：评估宫颈阴道液中HPV的存在与体外受精(IVF)结局的关系，以及选定的影响胚胎植入和妊娠的细胞因子：粒细胞-巨噬细胞集落刺激因子(GM-CSF)和粒细胞集落刺激因子(G-CSF)的浓度。研究设计：取卵当天采集82例妇女宫颈阴道标本。用50毫升无菌水冲洗阴道，收集液体3毫升。结果：HPV阳性12例(15%)。有趣的是，在HPV阳性的妇女中，活产率大约是HPV阳性妇女的一半，尽管由于病例数量较少，差异在统计学上并不显著。采用双抗体夹心法对29例(8例HPV阳性和21例HPV阴性)妇女宫颈阴道标本进行GM-CSF和G-CSF检测。HPV阳性妇女宫颈阴道液中GM-CSF水平显著低于HPV阳性妇女。结论：由于GM-CSF在妊娠过程中起重要作用，HPV阳性妇女宫颈阴道液中GM-CSF水平降低可能是导致HPV阳性妇女活产率降低的原因之一。(C)2013爱思唯尔B.V.保留所有权利。
Background: Although human papillomavirus (HPV) is the most common sexually transmitted infection, there are very scant data about the influence of this virus on the in vitro fertilization outcome.Objectives: To assess the presence of HPV in the cervico-vaginal fluid in relationship to the in vitro fertilization (IVF) outcome and to the concentration of selected cytokines, known to affect embryo implantation and gestation: granulocyte-macrophage colony stimulating factor (GM-CSF) and granulocyte colony stimulating factor (G-CSF).Study design: Cervico-vaginal samples were collected on the day of oocyte pick-up from 82 women. Vaginas were flushed with 50 mL of sterile water and 3 mL of fluid was collected.Results: Twelve women (15%) were positive for HPV. Interestingly, among HPV+ women live birth rate was about half of the rate in HPV- women, although the differences were not statistically significant due to the low number of cases. Cervico-vaginal samples of a sub-group of 29 (8 HPV+ and 21 HPV-) women were analyzed for GM-CSF and G-CSF by ELISA. GM-CSF but not G-CSF was significantly lower in the cervico-vaginal fluid of HPV+ than in HPV- women.Conclusions: Since GM-CSF plays an important role during pregnancy, the reduced levels of GM-CSF in the cervico-vaginal fluid of HPV+ women might contribute to explain the reduced live birth rate observed in HPV+ women. (C) 2013 Elsevier B.V. All rights reserved.