Targeted reduction of the DNA methylation level with 5-azacytidine promotes excision of the medaka fish Tol2 transposable element
Targeted reduction of the DNA methylation level with 5-azacytidine promotes excision of the medaka fish Tol2 transposable element
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DOI：
10.1017/s0016672306008184
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发表时间：
2006-06-01
期刊：
影响因子：
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通讯作者：
Koga, Akihiko
中科院分区：
文献类型：
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作者：
Iida, Atsuo;Shimada, Atsuko;Koga, Akihiko
青鳉属鱼类Oryzias latipes的Tol 2元件是hAT（流浪汉/Activator/Tam 3）转座因子家族的成员。有证据表明，在基因组和整个物种在过去的快速扩张，但高的自发转座率没有观察到与目前的鱼类材料，这表明Tol 2元件和它的宿主物种已经获得了一个相互作用的机制，以控制转座频率。DNA甲基化是一个可能的促成因素，因为它与许多其他转座因子有关。因此，我们将胚胎浸泡在5-氮杂胞苷中，这是一种导致DNA甲基化水平降低的试剂，并检查了反映体细胞切除频率的PCR产物的量，获得了暴露促进Tol 2切除的直接证据。因此，我们的研究结果表明，基因组DNA的甲基化是一个因素，包括在假定的控制转座的Tol 2元件的机制。
The Tol2 element of the medaka fish Oryzias latipes is a member of the hAT (hobo/Activator/Tam3) transposable element family. There is evidence for rapid expansion in the genome and throughout the species in the past but a high spontaneous transposition rate is not observed with current fish materials, suggesting that the Tol2 element and its host species have already acquired an interactive mechanism to control the transposition frequency. DNA methylation is a possible contributing factor, given its involvement with many other transposable elements. We therefore soaked embryos in 5-azacytidine, a reagent that causes reduction in the DNA methylation level, and examined amounts of PCR products reflecting the somatic excision frequency, obtaining direct evidence that exposure promotes Tol2 excision. Our results thus suggest that methylation of the genome DNA is a factor included in the Putative mechanisms of control of transposition of the Tol2 element.