The CCCH zinc finger protein gene AtZFP1 improves salt resistance in Arabidopsis thaliana
The CCCH zinc finger protein gene AtZFP1 improves salt resistance in Arabidopsis thaliana
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DOI：
10.1007/s11103-014-0226-5
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发表时间：
2014-10-01
影响因子：
5.1
通讯作者：
Wang, Baoshan
中科院分区：
文献类型：
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作者：
Han, Guoliang;Wang, Mingjie;Wang, Baoshan
CCCH型锌指蛋白是一个超家族，参与植物生长发育的许多方面。在本研究中，我们研究了拟南芥中一种 CCCH 型锌指蛋白 AtZFP1 (At2g25900) 对盐胁迫的响应。 AtZFP1 的表达因盐胁迫而上调。与转基因菌株相比，NaCl处理下野生植物的发芽率、子叶出现率和根长显着降低，而T-DNA插入突变株的抑制作用显着严重。发芽期氯化钠处理主要是渗透胁迫。相对于野生植物，过表达菌株保持了较高的K+、K+/Na+、叶绿素和脯氨酸含量，以及较低的Na+和MDA含量。实时定量PCR分析表明，盐胁迫下转基因菌株中胁迫相关标记基因KIN1、RD29B和RD22的表达量增加更为显着。 AtZFP1 的过表达还增强了氧化和渗透应激耐受性，这是通过测量一组抗氧化基因、渗透应激基因和离子转运蛋白基因（如 SOS1、AtP5CS1 和 AtGSTU5）的表达来确定的。总的来说，我们的结果表明 AtZFP1 的过度表达通过维持离子平衡和限制氧化和渗透应激来增强耐盐性。
The CCCH type zinc finger proteins are a super family involved in many aspects of plant growth and development. In this study, we investigated the response of one CCCH type zinc finger protein AtZFP1 (At2g25900) to salt stress in Arabidopsis. The expression of AtZFP1 was upregulated by salt stress. Compared to transgenic strains, the germination rate, emerging rate of cotyledons and root length of wild plants were significantly lower under NaCl treatments, while the inhibitory effect was significantly severe in T-DNA insertion mutant strains. At germination stage, it was mainly osmotic stress when treated with NaCl. Relative to wild plants, overexpression strains maintained a higher K+, K+/Na+, chlorophyll and proline content, and lower Na+ and MDA content. Quantitative real-time PCR analysis revealed that the expression of stress related marker genes KIN1, RD29B and RD22 increased more significantly in transgenic strains by salt stress. Overexpression of AtZFP1 also enhanced oxidative and osmotic stress tolerance which was determined by measuring the expression of a set of antioxidant genes, osmotic stress genes and ion transport protein genes such as SOS1, AtP5CS1 and AtGSTU5. Overall, our results suggest that overexpression of AtZFP1 enhanced salt tolerance by maintaining ionic balance and limiting oxidative and osmotic stress.