Urease of Corynebacterium glutamicum:: organization of corresponding genes and investigation of activity
Urease of Corynebacterium glutamicum:: organization of corresponding genes and investigation of activity
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DOI：
10.1111/j.1574-6968.2000.tb09248.x
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发表时间：
2000-08-15
影响因子：
2.1
通讯作者：
Burkovski, A
中科院分区：
文献类型：
--
作者：
Nolden, L;Beckers, G;Burkovski, A
谷氨酸棒杆菌编码尿素酶的基因被分离和测序。ureA、ureB和ureC是编码尿素酶结构亚基的蛋白，ureE、ureF、ureG和ureD是编码辅助蛋白的蛋白。从DNA序列分析推断，lire基因是转录偶联的，这至少在uveABC中被RT-PCR证明。基因破坏实验表明，结构蛋白（UreC）和辅助蛋白（UreD）是不可或缺的尿素酶活性和尿素上的生长。在各种培养基中生长后，测定不同棒状杆菌属物种中的尿素酶活性。虽然观察到的调控模式揭示了种属特异性差异，一般情况下，尿素酶活性诱导氮饥饿。与分枝杆菌一样，棒状杆菌中的尿素酶活性在致病物种中最高，并且也可能在宿主-病原体相互作用中发挥作用。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The Courynebacterium glutamicum genes encoding urease were isolated and sequenced. While ureA, ureB and ureC are encoding structural subunits of urease, ureE, ureF, ureG and ureD are encoding accessory proteins. As deduced from DNA sequence analyses, the lire genes are transcriptionally coupled, this was proven by RT-PCR at least for uveABC. Gene disruption experiments revealed that both structural (UreC) and accessory proteins (UreD) are indispensable for urease activity and growth on urea. Urease activity was determined in different Corynebacterium species after growth in various media. While the regulation patterns observed revealed species-specific differences, in general urease activity is induced upon nitrogen starvation. As in mycobacteria, in corynebacteria urease activity was highest in a pathogenic species and might also play a role in host-pathogen interaction. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.