Physical and biological evaluations of sintered hydroxyapatite/silicone composite with covalent bonding for a percutaneous implant material.
Physical and biological evaluations of sintered hydroxyapatite/silicone composite with covalent bonding for a percutaneous implant material.
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用于经皮植入材料的具有共价键合的烧结羟基磷灰石/有机硅复合材料的物理和生物学评估。
DOI：
10.1002/jbm.b.10002
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
A. Kishida
中科院分区：
文献类型：
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作者：
T. Furuzono;Pao‐Li Wang;A. Korematsu;K. Miyazaki;Mari Oido;Y. Kowashi;K. Ohura;J. Tanaka;A. Kishida
已开发出平均直径为2.0 μ m的羟基磷灰石（HA）微粒与硅胶基质共价连接的复合材料（HA/硅胶），并对其作为经皮软组织相容性材料的物理和生物学性能进行了评价。在拉伸性能测量中，HA/硅树脂和原始硅树脂的样品在拉伸强度上相似，约为100。7.8 MPa，断裂伸长率，约。百分之五百七十结果发现，化学表面改性与HA颗粒没有机械缺点。在胶带剥离试验中，扫描电子显微镜（SEM）观察表明，HA颗粒直接耦合到基板没有被删除。HA颗粒可以与基材牢固结合。在人牙周膜成纤维细胞附着和增殖实验中，孵育24 h后，附着于HA/硅胶的细胞数量是附着于原始硅胶的细胞数量的14倍。HA/硅胶上的值约为100。与用作阳性对照的组织培养塑料相比，80%。孵育72小时后，在HA/硅胶上生长的细胞数量增加至阳性对照水平。在Hoechst 33342染色的荧光显微镜观察中，由于细胞核形态完整，细胞似乎紧紧粘附在与硅胶片偶联的HA颗粒上。用罗丹明鬼笔环肽荧光染料观察细胞显示HA/硅胶上广泛的F-肌动蛋白细胞骨架。在为期4周的动物植入试验中，拉出HA/硅胶片所需的力是原始硅胶的15倍。HA-颗粒涂层的有机硅与共价键给出了惰性的表面生物活性。因此，HA复合物有效地防止了细菌感染。
A composite (HA/silicone) of hydroxyapatite (HA) microparticles with an average diameter of 2.0 micro m covalently linked to a silicone substrate has been developed, and its physical and biological properties as a percutaneous soft-tissue-compatible material have been evaluated. In tensile property measurement, samples of HA/silicone and the original silicone were similar in tensile strength, ca. 7.8 MPa, and elongation at break, ca. 570%. It was found that chemical surface modification with HA particles presented no mechanical disadvantage. In an adhesive-tape peeling test, scanning electron microscopic (SEM) observation showed that HA particles coupled directly to the substrate were not removed. HA particles may bond strongly with the substrate. In human periodontal ligament fibroblast attachment and proliferation experiments, the number of cells attached to HA/silicone was 14 times greater than that attached to the original silicone after 24 h of incubation. The value on HA/silicone was ca. 80% versus that on a tissue-culture plastic used as a positive control. After 72 h of incubation, the number of cells grown on HA/silicone increased to the level of the positive control. In observation of fluorescence microscopy stained by Hoechst 33342, cells appeared to tightly adhere to HA particles coupled to the silicone sheet due to intact nuclear morphology. Observation of cells by fluorescence dye with rhodamin phalloidin showed an extensive F-actin cytoskeleton on HA/silicone. In a 4-week animal implant test, force required to pull out the HA/silicone sheet was 15 times that of the original silicone. HA-particle coating on silicone with covalent linkage gave the inert surface bioactivity. The HA composite thus effectively prevents germ infection percutaneously.