ORDER OF ACTION OF COMPONENTS IN THE YEAST PHEROMONE RESPONSE PATHWAY REVEALED WITH A DOMINANT ALLELE OF THE STE11-KINASE AND THE MULTIPLE PHOSPHORYLATION OF THE STE7-KINASE
ORDER OF ACTION OF COMPONENTS IN THE YEAST PHEROMONE RESPONSE PATHWAY REVEALED WITH A DOMINANT ALLELE OF THE STE11-KINASE AND THE MULTIPLE PHOSPHORYLATION OF THE STE7-KINASE
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DOI：
10.1101/gad.6.7.1305
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发表时间：
1992-07-01
影响因子：
10.5
通讯作者：
KORNBERG, RD
中科院分区：
文献类型：
--
作者：
CAIRNS, BR;RAMER, SW;KORNBERG, RD
介导单倍体酵母细胞对肽交配信息素的反应的信号转导途径涉及包括蛋白激酶STE7和STE11的几个组分。我们已经分离和表征了STE11基因的显性等位基因，并且已经证明了STE11蛋白的氨基末端截短形式的表达引起交配途径的组成性激活。这种显性STE11等位基因的表达也恢复了某些不育株的交配能力。结合其他人的结果，我们的上位性结果建立了以下通路组分的作用顺序：STE2，GPA1（SCG1），STE4，STE5，STE11，STE7，STE12。从STE11到STE7的信号转导可能涉及磷酸化，因为STE7显示几种磷酸化形式，并且STE7响应于信息素或显性STE11蛋白的共表达而被多重磷酸化。进一步的信号传播似乎需要STE7蛋白激酶活性，因为催化受损的STE7突变体在交配反应中有缺陷。
The signal transduction pathway that mediates the response of haploid yeast cells to peptide mating pheromones involves several components including the protein kinases STE7 and STE11. We have isolated and characterized a dominant allele of the STE11 gene and have demonstrated that expression of an amino-terminally truncated form of STE11 protein causes constitutive activation of the mating pathway. Expression of this dominant STE11 allele also restored mating ability to certain sterile strains. In conjunction with the results of others, our epistasis results establish the following order of action of pathway components: STE2, GPA1(SCG1), STE4, STE5, STE11, STE7, STE12. Transduction of the signal from STE11 to STE7 may involve phosphorylation because STE7 displays several phosphorylation forms, and STE7 is multiply phosphorylated in response to either pheromone or coexpression of dominant STE11 protein. Further signal propagation appears to require STE7 protein kinase activity, because a catalytically impaired STE7 mutant is defective in the mating response.