Structural studies of the mineral phase of calcifying cartilage.
Structural studies of the mineral phase of calcifying cartilage.
复制标题
钙化软骨矿物相的结构研究。
DOI：
10.1002/jbmr.5650060514
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Glimcher,MJ
中科院分区：
文献类型：
--
作者：
Rey,C;Beshah,K;Griffin,R;Glimcher,MJ
通过X射线衍射、傅里叶变换红外光谱、魔角31 P核磁共振光谱和化学成分研究了小牛骨骺生长板的钙化软骨。通过密度离心分离粉末状组织，作为矿物质含量的函数，从而定性地确定钙-磷矿物质相的年龄。单独检查单个密度离心组分。样品的X射线衍射，特别是最低密度部分，显示出非常差的结晶磷灰石。傅立叶变换红外光谱和31 P核磁共振光谱显示存在显着量的非磷灰石磷酸根离子。这种nonapatitic磷酸盐的浓度在矿化的早期阶段增加，但随后随着矿物质含量的稳步上升而降低，直到完全矿化。碳酸根离子的总浓度在钙化软骨中比在来自同一器官（肩胛骨）的骨中低得多。碳酸根离子位于A位（OH−）和B位（PO 43-），其分布与骨矿物中的分布相似，但磷酸根和碳酸根红外光谱的分辨因子之间的差异与磷酸钙固相中碳酸根离子在组织差的区域中的不均匀分布一致。这些初步的研究使人们能够将磷酸钙矿物相表征为结晶性非常差的未成熟磷酸钙磷灰石，富含不稳定的非磷灰石磷酸根离子，与同一动物的骨矿物相比，碳酸根离子浓度低，实际上来自同一器官（肩胛骨）的骨。
The calcified cartilage of the epiphyseal growth plate of young calves has been studied by x‐ray diffraction, Fourier transform infrared spectroscopy, magic angle31P nuclear magnetic resonance spectroscopy, and chemical composition. The powdered tissue was separated by density centrifugation as a function of mineral content and thus qualitatively of the age of the calcium‐phosphorus mineral phase. The individual density centrifugation fractions were examined separately. X‐ray diffraction of the samples, especially of the lowest density fractions, revealed very poorly crystalline apatite. Fourier transform infrared spectroscopy and31P nuclear magnetic resonance spectroscopy revealed the presence of significant amounts of nonapatitic phosphate ions. The concentration of such nonapatitic phosphates increases during the early stages of mineralization but then decreases as the mineral content steadily rises until full mineralization is achieved.The total concentration of carbonate ions was found to be much lower in calcified cartilage than in bone from the same organ (scapula). The carbonate ions are located in both A sites (OH−) and B sites (PO43‐), with a distribution similar to that found in bone mineral. However, discrepancies between infrared resolution factors of phosphate and carbonate bands are consistent with a heterogeneous distribution of carbonate ions in poorly organized domains of the solid phase of calcium phosphate. These initial studies permit one to characterize the calcium phosphate mineral phase as a very poorly crystalline, immature calcium phosphate apatite, rich in labile nonapatitic phosphate ions, with a low concentration of carbonate ions compared with bone mineral of the same animal, indeed from the bone of the same organ (scapula).