Intrauterine Growth Restriction Modifies the Developmental Pattern of Intestinal Structure, Transcriptomic Profile, and Bacterial Colonization in Neonatal Pigs
Intrauterine Growth Restriction Modifies the Developmental Pattern of Intestinal Structure, Transcriptomic Profile, and Bacterial Colonization in Neonatal Pigs
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DOI：
10.3945/jn.109.116822
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发表时间：
2010-05-01
影响因子：
4.2
通讯作者：
Le Huerou-Luron, Isabelle
中科院分区：
文献类型：
--
作者：
D'Inca, Romain;Kloareg, Maela;Le Huerou-Luron, Isabelle
胎儿宫内生长受限（IUGR）的新生儿易患消化系统疾病。使用IUGR的新生猪，我们测试的假设，IUGR可能会引起肠道屏障的发育模式的改变，从而可能是负责IUGR相关的发病率增加。将出生体重接近平均出生体重（+/-0.5 SD）的仔猪确定为正常出生体重（对照），将出生体重平均低于-2 SD（-30%）的仔猪定义为IUGR仔猪。从出生到出生后第5天，研究了肠道结构、转录谱和细菌定植的发育模式。出生时，IUGR仔猪的肠重和肠长、单位长度回肠和结肠重以及绒毛大小均低于同龄对照仔猪（P < 0.05）。这些IUGR诱导的肠道改变进一步持续，虽然他们在第5天不太明显。2日龄IUGR仔猪回肠和结肠粘膜粘附菌数显著高于同龄对照仔猪（P < 0.05）。对肠道转录组谱的动态分析揭示了IUGR诱导的肠道生长障碍的分子证据，这可能是由于生命最初几天细胞增殖-凋亡平衡的变化，而保护过程将在以后发生。几种基因表达的变化表明，糖皮质激素和微生物群在IUGR肠发育过程中起着关键作用。新生儿期J.营养140：925-931，2010.
Neonates with intrauterine growth restriction (IUGR) are prone to suffer from digestive diseases. Using neonatal pigs with IUGR, we tested the hypothesis that IUGR may induce alterations in the developmental pattern of intestinal barrier and thereby may be responsible for IUGR-associated increased morbidity. Piglets with a birth weight near the mean birth weight (+/- 0.5 SD) were identified as normal birth weight (control) and piglets with a mean -2 SD lower birth weight (-30%) were defined as piglets with IUGR. The developmental pattern of intestinal structure, transcriptornic profile, and bacterial colonization was investigated from birth to d 5 postnatal. At birth, intestinal weight and length, ileal and colonic weight per unit of length, and villous sizes were lower (P < 0.05) in piglets with IUGR than in same-age control piglets. These IUGR-induced intestinal alterations further persisted, although they were less marked at d 5. Counts of adherent bacteria to ileal and colonic mucosa were greater (P < 0.05) in 2-d-old piglets with IUGR than in same-age control piglets. Dynamic analyses of the transcriptomic profile of the intestine revealed molecular evidence of IUGR-induced intestinal growth impairment that may result from a change in the cell proliferation-apoptosis balance during the first days of life, while a protective process would occur later on. In addition, changes in the expression of several genes suggest a pivotal role of both glucocorticoids and microbiota in driving IUGR intestinal development during the neonatal period. J. Nutr. 140: 925-931, 2010.