Radioimmunoassay of vasopressin in unextracted plasma.
Radioimmunoassay of vasopressin in unextracted plasma.
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未提取血浆中加压素的放射免疫测定。
DOI：
10.3109/00365517609081947
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发表时间：
1976
影响因子：
2.1
通讯作者：
H. Hollemans
中科院分区：
文献类型：
--
作者：
F. Fyhrquist;M. Wallenius;H. Hollemans
未提取的人血浆中精氨酸加压素（AVP）的放射免疫测定（RIA）是基于敏感的抗AVP兔抗血清，抑制[125 I]AVP和AVP的酶损伤，并使用单个血浆空白，以校正血浆因素对RIA的干扰。检测到的合成AVP的灵敏度为0.4 pg，对应于人血浆中1.2 pg/ml的AVP。加入合并血浆的AVP回收率为94 +/- 9.3%（平均值+/- S.D.）在低范围（AVP，添加2.8pg/ml）中为106 +/-11.7%，在高范围（添加45.0pg/ml）中为106 +/-11.7%。在26名自由活动的健康受试者中，水摄入量，仰卧位血浆AVP浓度为2.0 +/- 1.22 pg/ml，在28名健康受试者中，直立位血浆AVP浓度为6.2 +/- 4.3 pg/ml。水负荷抑制血浆AVP浓度。吸烟引起3例受试者血浆AVP升高，尽管水负荷。
A radioimmunoassay (RIA) for arg8-vasopressin (AVP) in unextracted human plasma was based on a sensitive anti-AVP rabbit antiserum, inhibition of enzymatic damage to [125I]AVP and AVP, and the use of an individual plasma blank, to correct for interference of plasma factors with the RIA. Sensitivity was 0.4 pg of synthetic AVP detected, corresponding to 1.2 pg/ml of AVP in human plasma. Recovery of AVP added to pooled plasma was 94 +/- 9.3% (mean +/- S.D.) in the low range (AVP, 2.8 pg/ml added) and 106 +/- 11.7% in the high range (45.0 pg/ml added). In 26 healthy, ambulatory subjects on ad lib, water intake, plasma AVP concentration was 2.0 +/- 1.22 pg/ml in the supine position and in 28 healthy subjects, 6.2 +/- 4.3 pg/ml in the upright position. Water loading suppressed the plasma AVP concentration. Smoking caused increased plasma AVP in 3 subjects despite water loading.