Hybridisation between wolves and dogs in Latvia as documented using mitochondrial and microsatellite DNA markers
Hybridisation between wolves and dogs in Latvia as documented using mitochondrial and microsatellite DNA markers
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DOI：
10.1078/1616-5047-00012
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发表时间：
2002-01-01
影响因子：
1.6
通讯作者：
Ozolins, J
中科院分区：
文献类型：
--
作者：
Andersone, Z;Lucchini, V;Ozolins, J
野生狼和狗的杂交有时会被报道，但在科学文献中总是很少有记载。然而，记录杂交和导入的频率对于保护野生生活的狼种群和管理自由放养的狗很重要。本文报道了1997年至1999年在拉脱维亚采集的31个狼样本的分子遗传学分析结果，其中包括1999年3月在拉脱维亚北部发现的6只幼崽，以及6只狼的形态特征，表明它们可能是杂交起源的。线粒体DNA控制区高变区的核苷酸序列测定和16个微卫星座位的基因分型表明，幼崽和形态异常的狼都可能起源于与狗的杂交。狼狗杂交的原因，以及可能的管理努力，以避免在野外进一步杂交，进行了讨论。
Crossbreeding between wolves and dogs in the wild has been sometimes reported, but always poorly documented in scientific Literature. However, documenting frequency of hybridisation and introgression is important for conservation of wild living wolf populations and for the management of free ranging dogs. Here we report the results of molecular genetic analyses of 31 wolf samples collected in Latvia from 1997 to 1999, including six pups originated from a litter found in northern Latvia in March 1999, and six wolves showing morphological traits that suggested hybrid origin. Nucleotide sequencing of the hypervariable part of the mtDNA control-region and genotyping of 16 microsatellite loci suggested that both pups and the morphologically anomalous wolves might originate from crossbreeding with dogs. Causes of wolf-dog crossbreeding, as well as possible management effort to avoid further hybridisation in the wild, are discussed.