A UV-induced mutation in neurospora that affects translational regulation in response to arginine.
A UV-induced mutation in neurospora that affects translational regulation in response to arginine.
复制标题
神经孢子菌中紫外线诱导的突变，影响对精氨酸的翻译调节。
DOI：
10.1093/genetics/142.1.117
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Sachs,MS
中科院分区：
文献类型：
--
作者：
Freitag,M;Dighde,N;Sachs,MS
链孢菌crmsu arg-2基因编码丝氨酸特异性氨甲酰磷酸合成酶的小亚基。精氨酸对arg-2 mRNA水平和mRNA翻译水平有负调节作用。转录本5′区的上游开放阅读框（uORF）与丝氨酸特异性调控有关。将编码潮霉素磷酸转移酶的Anarg-2-hph融合基因导入N.我们使用anarg-2-hph菌株来选择在潮霉素和精氨酸存在下生长的UV诱导的突变体，并且我们分离了46个具有两种表型中的任一种的突变体。其中一个表型表明arg-2-hph和urg-2基因表达均发生改变，另一个表型表明urg-2-hph和urg-2基因表达均发生改变。序列分析和转化实验揭示了uORF密码子12处的突变（Asp至Asn），其废除了负调控。核糖体onarg-2-hph转录本的分布检查表明，失去监管有一个翻译组件，表明uORF序列是重要的精氨酸特异性翻译控制。与其他uORFS的比较表明，在翻译控制机制的共同元素。
TheNeurospora crmsu arg-2gene encodes the small subunit of arginine-specific carbamoyl phosphate synthetase. The levels ofarg-2mRNA and mRNA translation are negatively regulated by arginine. An upstream open reading frame (uORF) in the transcript’s 5′ region has been implicated in arginine-specific control. Anarg-2-hphfusion gene encoding hygromycin phosphotransferase conferred arginine-regulated resistance to hygromycin when introduced intoN. crassa.We used anarg-2-hphstrain to select for UV-induced mutants that grew in the presence of hygromycin and arginine, and we isolated 46 mutants that had either of two phenotypes. One phenotype indicated altered expression of botharg-2-hphandurg-2genes; the other, altered expression ofurg-2-hphbut notarg-2.One of the latter mutations, which was genetically closely linked toarg-2-hph,was recovered from the 5′ region of thearg-2-hphgene using PCR. Sequence analyses and transformation experiments revealed a mutation at uORF codon 12 (Asp to Asn) that abrogated negative regulation. Examination of the distribution of ribosomes onarg-2-hphtranscripts showed that loss of regulation had a translational component, indicating the uORF sequence was important for Arg-specific translational control. Comparisons with other uORFS suggest common elements in translational control mechanisms.