The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza
The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza
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DOI：
10.1016/s0141-0229(00)00284-2
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发表时间：
2001-01-02
影响因子：
3.4
通讯作者：
Lo, CMY
中科院分区：
文献类型：
--
作者：
Chen, H;Chen, F;Lo, CMY
发根农杆菌ATCC 15834转化的丹参毛状根培养物在正常生长条件下产生微量的丹参酮和酚酸成分水平。经酵母诱导子诱导后，酚酸和丹参酮的产量均得到提高。其中迷迭香酸和紫草酸B含量分别从1.24%和2.59%提高到2.89%和2.98%，隐丹参酮含量从0.001%提高到0.096%。酵母诱导子还改善了毛状根的生长（基于干重从3.9g/l至7.3g/l）。采用液相色谱-质谱联用（LC-MS）法同时检测和鉴定丹参提取物中的酚酸类和丹参酮类成分。丹参。通过与标准品比较，鉴别了迷迭香酸、紫草酸B、隐丹参酮、丹参酮I、丹参酮IIA和丹参酮II B。通过分子量和洗脱度与文献相当，初步鉴定了二氢丹参酮I和亚甲基丹参醌。在酵母诱导子处理的毛状根培养物中发现了一种分子量为280的未知化合物，它是诱导的主要丹参酮之一。(C)2001 Elsevier Science Inc. All rights reserved.
Hairy root cultures of Salvia miltiorrhiza transformed with Agrobacterium rhizogenes ATCC 15834 produced a tiny amount of tanshinones and a constituent level of phenolic acids under normal growth conditions. Upon elicitation with yeast elicitor, the production of both phenolic acids and tanshinones was enhanced. For example, the contents of two phenolic acids, rosmarinic acid and lithospermic acid B were elevated from 1.24% and 2.59% to 2.89% and 2.98% of dry wt, respectively while the intracellular content of cryptotanshinone increased from 0.001% to as much as 0.096% of dry wt. Yeast elicitor also improved the growth of hairy roots (from 3.9 g/l to 7.3 g/l on a dry wt basis). Liquid chromatography-mass spectrometry (LC-MS) was developed for simultaneous detection and identification of phenolic acids and tanshinones in the extracts of S. miltiorrhiza. Rosmarinic acid, lithospermic acid B, cryptotanshinone, tanshinone I, tanshinone IIA and tanshinone IIB were identified by comparison with standards available. Dihydrotanshinone I and methylenetanshiquinone were tentatively identified by the molecular weights and the elution comparable with the literature. An unknown compound with a molecular weight of 280 was found in yeast-elicitor treated hairy root cultures, which was one of the major tanshinones induced. (C) 2001 Elsevier Science Inc. All rights reserved.