Somatic diversification of S107 from an antiphosphocholine to an anti-DNA autoantibody is due to a single base change in its heavy chain variable region.
Somatic diversification of S107 from an antiphosphocholine to an anti-DNA autoantibody is due to a single base change in its heavy chain variable region.
复制标题
S107 从抗磷酸胆碱抗体到抗 DNA 自身抗体的体细胞多样化是由于其重链可变区的单个碱基变化所致。
DOI：
10.1073/pnas.84.9.2926
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发表时间：
1987
影响因子：
11.1
通讯作者：
Scharff,MD
中科院分区：
文献类型：
--
作者：
Giusti,AM;Chien,NC;Zack,DJ;Shin,SU;Scharff,MD
S107骨髓瘤细胞系表达T15抗磷酸胆碱（P-Cho）抗体的种系序列，该抗体是BALB/c小鼠对P-Cho产生应答的主要抗体，无论是在各种细菌多糖上还是在附着于蛋白质载体时。我们以前曾报道，S107细胞系的体细胞突变体产生的抗体已经失去了结合P-Cho的能力，并获得了双链DNA的结合。该抗体在重链可变区的残基35处用丙氨酸取代谷氨酸。我们现在表明，这种氨基酸取代是由于一个单一的A-C颠换，这是重链和轻链可变区中唯一的核苷酸变化。此外，这种变化似乎是由于体细胞突变而不是基因转换。
The S107 myeloma cell line expresses the germ-line sequence of the T15 antiphosphocholine (P-Cho) antibody, which is the major antibody made by BALB/c mice in response to P-Cho, either on a variety of bacterial polysaccharides or when attached to a protein carrier. We have previously reported that a somatic mutant of the S107 cell line produces an antibody that has lost the ability to bind P-Cho and has acquired binding for double-stranded DNA. This antibody has a substitution of an alanine for a glutamic acid at residue 35 in the heavy chain variable region. We now show that this amino acid substitution is due to a single A-C transversion, which is the only nucleotide change in the heavy and light chain variable regions. Further, it appears that this change is due to somatic mutation rather than to gene conversion.