Bradykinin induces the bi-phasic production of lysophosphatidyl inositol and diacylglycerol in a dorsal root ganglion X neurotumor hybrid cell line, F-11.
Bradykinin induces the bi-phasic production of lysophosphatidyl inositol and diacylglycerol in a dorsal root ganglion X neurotumor hybrid cell line, F-11.
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缓激肽诱导背根神经节 X 神经肿瘤杂交细胞系 F-11 中溶血磷脂酰肌醇和二酰甘油的双相产生。
DOI：
10.1016/s0006-291x(88)80098-6
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发表时间：
1988
影响因子：
3.1
通讯作者：
Dawson,G
中科院分区：
文献类型：
--
作者：
Francel,P;Dawson,G
缓激肽作用于背根神经节X神经母细胞瘤杂交细胞系F-11，刺激三磷酸肌醇（IP 3）和细胞内钙的快速升高。我们现在显示花生四烯酸标记的二酰基甘油（DAG）的同样快速的释放（对照的243±32%）。用200 ng/ml百日咳毒素预处理过夜或用10 nM十四酰佛波醇醋酸酯（TPA）可抑制二酰甘油产生的第一个峰值。此外，发生了第二次更持续的释放，在约5分钟时达到稳定（304±16%）。DAG的第二个峰不受这些TPA或百日咳预孵育的影响。肌醇标记磷脂的同时分析表明，初始的IP 3和DAG峰对应于磷酸肌醇PIP 2和PIP的初始减少，而PI在同一时间段内略有增加。相反，在5-30分钟，PIP 2和PIP恢复到正常水平，但PI逐渐下降到对照值的75%。同样，TPA阻断了这种早期PIP和PIP 2的分解，但对单磷脂酰肌醇（PI）的延迟分解没有影响。缓激肽也诱导溶血磷脂酰肌醇（lyso-PI）同样快速的增加，在10-30秒左右达到峰值，10分钟后出现第二个更持续的峰值。这种溶血PI的产生不受TPA或百日咳毒素预先处理的影响。甘油二酯生产的初始阶段和持续阶段可能是由不同的生化机制和/或底物引起的。
Bradykinin acts on the dorsal root ganglion X neuroblastoma hybrid cell line F-11 to stimulate the rapid elevation of inositol trisphosphate (IP3) and intracellular calcium. We now show an equally rapid release of arachidonyl labeled diacylglycerol (DAG), (243±32% of control). This first peak of diacylglycerol production was inhibitable by either pretreatment with 200 ng/ml of pertussis toxin overnight or by 10 nM tetradecanoylphorbol acetate (TPA). In addition, a second, more sustained release occurred, plateauing at approximately five minutes (304±16%). The second peak of DAG was unaffected by these TPA or pertussis pre-incubations. Simultaneous analysis of inositol-labeled phospholipids showed that the initial IP3and DAG peaks corresponded to initial decreases in phosphoinositides PIP2and PIP whereas PI increased slightly over this same time period. In contrast, at 5–30 minutes, PIP2and PIP returned to normal levels, but PI gradually decreased to 75% of control values. Likewise, TPA blocked this early PIP and PIP2breakdown, but had no effect on the delayed breakdown of monophosphatidylinositol (PI).Bradykinin also induced an equally rapid increase in lysophosphatidyl inositol (lyso-PI) with a peak around 10–30 seconds, and a second more sustained peak after 10 minutes. This production of lyso-PI was not affected by prior treatment with TPA or pertussis toxin. The initial and the sustained phases of diacylglycerol production probably result from different biochemical mechanisms and/or substrates.